| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIMM10B |
| Uniprot No | Q9Y5J6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-103aa |
| 氨基酸序列 | MERQQQQQQQLRNLRDFLLVYNRMTELCFQRCVPSLHHRALDAEEEACLHSCAGKLIHSNHRLMAAYVQLMPALVQRRIADYEAASAVPGVAAEQPGVSPSGS |
| 预测分子量 | 27.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TIMM10B重组蛋白的模拟参考文献示例(仅供参考，建议通过学术数据库验证具体文献)：
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1. **文献名称**：*Recombinant expression and functional characterization of human TIMM10B in mitochondrial protein import*
**作者**：Koehler, C.M., et al.
**摘要**：研究报道了人源TIMM10B重组蛋白在大肠杆菌中的表达与纯化，证实其在线粒体膜间隙中与TIMM10A形成复合物，协助疏水蛋白底物的转运，并分析了其突变体对功能的影响。
2. **文献名称**：*Structural insights into the TIMM10B-TIMM10A complex by X-ray crystallography*
**作者**：Bauer, M.F., et al.
**摘要**：通过X射线晶体学解析了TIMM10B与TIMM10A的复合物结构，揭示了其锌指结构域在底物识别中的作用，为线粒体蛋白质导入机制提供了分子基础。
3. **文献名称**：*TIMM10B deficiency disrupts mitochondrial homeostasis and induces apoptosis in cancer cells*
**作者**：Jin, S., et al.
**摘要**：研究发现，TIMM10B敲低导致线粒体膜电位下降及细胞色素C释放，重组TIMM10B可挽救细胞凋亡表型，表明其在维持线粒体功能中的关键作用。
4. **文献名称**：*Functional interplay between TIMM10B and mitochondrial chaperones during stress response*
**作者**：Chacinska, A., et al.
**摘要**：探讨了重组TIMM10B与HSP70等分子伴侣的协同作用，证明其在氧化应激条件下对错误折叠蛋白的线粒体导入具有保护功能。
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**注意**：以上内容基于领域知识模拟，具体文献需通过PubMed、Web of Science等平台检索确认。
TIMM10B (Translocase of Inner Mitochondrial Membrane 10B) is a key component of the mitochondrial TIM10 chaperone complex, which plays a critical role in protein import into mitochondria. As part of the TIM machinery, TIMM10B facilitates the translocation of nuclear-encoded proteins with α-helical transmembrane domains from the mitochondrial intermembrane space to the inner membrane. This process is essential for maintaining mitochondrial integrity, energy production, and metabolic regulation.
Structurally, TIMM10B belongs to the conserved mitochondrial small TIM chaperone family, characterized by a twin CX3C motif coordinating zinc ions to stabilize its folding. It forms a hexameric complex with TIMM9 and TIMM10A, acting as a molecular chaperone to prevent aggregation of hydrophobic substrate proteins during transport. Unlike other TIM components, TIMM10B shows tissue-specific expression patterns, with higher levels observed in metabolically active organs like the heart, liver, and brain.
Recombinant TIMM10B protein is typically produced using bacterial expression systems (e.g., E. coli) with affinity tags for purification. Its recombinant form enables detailed studies of mitochondrial protein import mechanisms, chaperone-substrate interactions, and structural analysis through techniques like X-ray crystallography or cryo-EM. Researchers also use it to investigate mitochondrial dysfunction linked to neurodegenerative diseases (e.g., Parkinson's and Alzheimer's), cancer metabolism abnormalities, and rare genetic disorders involving TIM complex deficiencies. Recent studies suggest its potential as a biomarker for mitochondrial stress and a therapeutic target for metabolic syndrome interventions.
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