| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEGP12 |
| Uniprot No | Q9LK70 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-499aa |
| 氨基酸序列 | SRIATIVLPFALTRGRKIHTMSKDEEWWKKIRKSPPVDELMLESVVEVFTDSTKYSKVKPWQTLNQESYGGSGFAIAGKKILTNAHVVEGMNDHIFVHVKRHGSQVKYKAKVQKIAHECDLAILEIDSDEFWKGMNPLEFGDIPPLNEIVYVVGYPKAGETICVTKGVVTGVKTGNYLRSSTKLLTIHIDATTYGGNSGGPVITGDKVLGVLFQILGDKKSTGVVIPTPIIRHFITGAEESSHNAVFGSLVLSCQSMKNAQIRNHFKMSPETTGILINKINSSSGAHKILRKDDIILAIDGVPVLSEMRRISFNHFISMKKPDENILVKVLRKGKEHEYNISLKPVKPHIQVQQYYNLPSYYIFGGFVFVPLTKSYIDDKYYKITDEQHVIISQVMPDDINKGYSNFKDLQVEKVNGVKVKNLKHLRELIEGCFSKDLRLDLENDKVMVLNYESAKKATFEILERHNIKSAWASE |
| 预测分子量 | 69.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DEGP12重组蛋白的虚构参考文献示例(基于常见研究方向的合理推测，实际文献需通过学术数据库验证)：
1. **文献名称**：*Recombinant Expression and Functional Characterization of DEGP12 in Bacterial Stress Response*
**作者**：Chen L, et al.
**摘要**：研究DEGP12在大肠杆菌中的重组表达，证实其作为分子伴侣和丝氨酸蛋白酶的双重功能，分析其在高温应激下对错误折叠蛋白的降解机制。
2. **文献名称**：*Structural Insights into the Activation Mechanism of DEGP12 Protease*
**作者**：Wang Y, et al.
**摘要**：通过冷冻电镜解析DEGP12的三维结构，揭示其底物结合后从静息态到活化态的构象变化，阐明其自激活的分子基础。
3. **文献名称**：*DEGP12-Mediated Mitochondrial Protein Quality Control in Neurodegenerative Disease Models*
**作者**：Kim S, et al.
**摘要**：在哺乳动物细胞中表达重组DEGP12.证明其通过清除线粒体内聚集蛋白减轻帕金森病模型的神经退行性病理表型。
4. **文献名称**：*Engineering Thermostable DEGP12 Variants for Industrial Enzyme Applications*
**作者**：Müller F, et al.
**摘要**：通过定向进化获得热稳定性增强的DEGP12突变体，验证其在高温环境下对底物蛋白的高效水解能力，拓展其在生物催化中的应用。
**注意**：以上内容为模拟示例，DEGP12相关研究可能与实际文献存在差异。建议结合具体研究背景，通过PubMed或Web of Science等平台检索准确信息。若名称有误(如DegP/HtrA家族成员)，可调整关键词重新查询。
**Background of DEGP12 Recombinant Protein**
DegP12. a member of the HtrA (High-temperature requirement A) protease family, is a multifunctional recombinant protein that combines chaperone-like activity with serine protease functionality. Originally derived from bacterial systems (e.g., *Escherichia coli*), DegP12 is engineered to address protein quality control by selectively degrading misfolded proteins or assisting in their proper folding under stress conditions. Its structure typically includes an N-terminal protease domain and two C-terminal PDZ domains, which regulate substrate recognition and allosteric activation.
Recombinant DegP12 is produced via heterologous expression in hosts like *E. coli* or mammalian cell lines, enabling scalable purification for research and industrial applications. Its dual role as a chaperone and protease is pH- and temperature-dependent: at lower temperatures or neutral pH, it predominantly acts as a folding assistant, while elevated stress triggers proteolytic activity to eliminate irreparable proteins. This adaptability makes it valuable in biopharmaceutical production, where it enhances yields of soluble, functional recombinant proteins by minimizing aggregation.
In biomedical research, DegP12 is utilized to study protein homeostasis mechanisms, particularly in diseases linked to protein misfolding (e.g., Alzheimer’s, Parkinson’s). Its engineered variants, such as catalytic mutants or PDZ domain truncations, further allow dissection of structure-function relationships. Industrial applications include optimizing enzyme production and improving microbial cell factories.
Overall, DegP12 exemplifies a versatile tool in both basic science and biotechnology, bridging protein engineering with therapeutic and industrial innovation.
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