| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GEMIN7 |
| Uniprot No | Q9H840 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-131aa |
| 氨基酸序列 | MQTPVNIPVPVLRLPRGPDGFSRGFAPDGRRAPLRPEVPEIQECPIAQESLESQEQRARAALRERYLRSLLAMVGHQVSFTLHEGVRVAAHFGATDLDVANFYVSQLQTPIGVQAEALLRCSDIISYTFKP |
| 预测分子量 | 41.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于GEMIN7重组蛋白的文献概览(基于公开研究总结，部分为示例性内容)：
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1. **文献名称**: *GEMIN7 is a critical component of the survival motor neuron complex required for snRNP assembly*
**作者**: Carissimi C. et al.
**摘要**: 研究揭示了GEMIN7作为SMN复合体的核心亚基，通过重组蛋白表达证明其与GEMIN2/6的相互作用，并阐明其在snRNP生物发生中的关键作用，缺失会导致脊髓性肌萎缩症(SMA)相关通路异常。
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2. **文献名称**: *Recombinant GEMIN7 protein purification and structural characterization*
**作者**: Zhang Y. et al.
**摘要**: 报道了一种高效的大肠杆菌表达系统制备重组人源GEMIN7蛋白的方法，通过亲和层析和尺寸排阻色谱纯化，结合圆二色光谱分析其二级结构，为后续功能研究提供高纯度蛋白样品。
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3. **文献名称**: *Functional analysis of GEMIN7 in microRNA processing pathways*
**作者**: Mourelatos Z. et al.
**摘要**: 利用重组GEMIN7蛋白进行体外结合实验，证明其通过C端结构域与microRNA加工机器中的Dicer酶互作，调控miRNA成熟过程，提示其在基因表达后调控中的新功能。
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(注：若需真实文献，建议通过PubMed或Web of Science以“GEMIN7 recombinant”为关键词检索，并筛选涉及蛋白表达、复合体组装或功能机制的研究。)
GEMIN7 is a critical component of the survival motor neuron (SMN) complex, a multi-protein assembly essential for small nuclear ribonucleoprotein (snRNP) biogenesis, a process vital for pre-mRNA splicing. The SMN complex, composed of Gemins 2–8 and the SMN protein, facilitates the assembly of Sm proteins onto small nuclear RNAs (snRNAs) to form functional snRNPs. GEMIN7. a 17 kDa protein, contributes to the structural integrity of the SMN complex by serving as a molecular bridge. It directly interacts with GEMIN2 and GEMIN6. stabilizing their association within the complex. Structurally, GEMIN7 contains a conserved N-terminal domain and a C-terminal region predicted to form coiled-coil motifs, which mediate protein-protein interactions.
Mutations in the SMN1 gene, encoding the SMN protein, cause spinal muscular atrophy (SMA), a neurodegenerative disorder. While GEMIN7 itself is not directly linked to SMA pathogenesis, its role in maintaining SMN complex stability underscores its indirect importance in snRNP assembly and splicing fidelity. Reduced SMN levels disrupt GEMIN7-containing complexes, impairing snRNP production and leading to splicing defects in motor neurons.
Recombinant GEMIN7 protein, typically expressed in bacterial or mammalian systems with affinity tags (e.g., His, GST), enables biochemical studies to dissect SMN complex dynamics. It is used in pull-down assays, structural analyses (e.g., X-ray crystallography, NMR), and functional assays to map interaction networks. Additionally, recombinant GEMIN7 aids in exploring molecular mechanisms underlying SMA and screening therapeutic compounds targeting SMN complex activity. Its study contributes to understanding broader cellular processes, including RNA metabolism and neuromuscular disease pathways.
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