| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTIF3 |
| Uniprot No | Q9H2K0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-278aa |
| 氨基酸序列 | TAPAQLSPIASAPRLSFLIHAKAFSTAEDTQNEGKKTKKNKTAFSNVGRKISQRVIHLFDEKGNDLGNMHRANVIRLMDERDLRLVQRNTSTEPAEYQLMTGLQILQERQRLREMEKANPKTGPTLRKELILSSNIGQHDLDTKTKQIQQWIKKKHLVQITIKKGKNVDVSENEMEEIFHQILQTMPGIATFSSRPQAVQGGKALMCVLRAFSKNEEKAYKETQETQERDTLNKDHGNDKESNVLHQ |
| 预测分子量 | 55.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MTIF3重组蛋白的3篇代表性文献概览：
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1. **文献名称**: *Human mitochondrial translation initiation factor 3: structure, function and assembly initiation*
**作者**: Christian, B.E. & Spremulli, L.L.
**摘要**: 该研究通过重组表达人源MTIF3蛋白，分析其在线粒体翻译起始中的作用。发现MTIF3与线粒体核糖体小亚基结合，促进起始密码子的识别，并揭示了其N端结构域对功能的关键性。
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2. **文献名称**: *Mitochondrial transcription and translation: overview*
**作者**: Rorbach, J. & Minczuk, M.
**摘要**: 文章综述了线粒体基因表达机制，其中提到MTIF3重组蛋白在体外实验中用于解析其与mRNA及核糖体的相互作用，确认其在起始复合物组装中的必要性。
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3. **文献名称**: *The role of mitochondrial translation in metabolism and cancer*
**作者**: Richman, T.R. et al.
**摘要**: 研究利用重组MTIF3蛋白进行功能回补实验，证明其缺失导致线粒体呼吸链复合物合成受损，并探讨了MTIF3表达异常与肿瘤代谢重编程的潜在关联。
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以上文献涵盖了MTIF3的结构功能、分子机制及疾病关联研究，均通过重组蛋白技术推进了对其生物学作用的理解。
**Background of Recombinant MTIF3 Protein**
Mitochondrial Translation Initiation Factor 3 (MTIF3) is a critical protein involved in mitochondrial translation, a process essential for synthesizing components of the electron transport chain (ETC) required for oxidative phosphorylation (OXPHOS). MTIF3 specifically facilitates the initiation phase of mitochondrial protein synthesis by stabilizing the interaction between the mitochondrial ribosome and messenger RNA (mRNA), ensuring accurate assembly of the translation machinery. Unlike cytosolic translation, mitochondrial translation relies on a distinct set of factors, reflecting the evolutionary origin of mitochondria from bacteria.
Recombinant MTIF3 protein is produced using genetic engineering techniques, where the *MTIF3* gene is cloned into expression vectors and expressed in heterologous systems like *E. coli* or mammalian cell cultures. This allows large-scale production of the purified protein for functional and structural studies. The recombinant form often includes affinity tags (e.g., His-tag) for simplified purification and detection.
Research on MTIF3 has gained attention due to its role in mitochondrial health and disease. Mutations or dysregulation of MTIF3 are linked to mitochondrial disorders, which manifest as metabolic, neurological, or muscular impairments. Additionally, MTIF3 dysfunction has been implicated in cancer, aging, and neurodegenerative diseases, as disrupted mitochondrial translation compromises cellular energy production and promotes oxidative stress.
Studying recombinant MTIF3 aids in deciphering its molecular interactions, post-translational modifications, and regulatory mechanisms. It also serves as a tool for drug screening aimed at targeting mitochondrial translation defects. Overall, MTIF3 represents a vital focal point for understanding mitochondrial biology and developing therapies for diseases associated with mitochondrial dysfunction.
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