| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Cltrn |
| Uniprot No | Q9ESG4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 15-141aa |
| 氨基酸序列 | ELCHPDAENAFKVRLSIRAALGDKAYVWDTDQEYLFRAMVAFSMRKVPNREATEISHVLLCNITQRVSFWFVVTDPSNNYTLPAAEVQSAIRKNRNRINSAFFLDDHTLEFLKIPSTLAPPMEPSVP |
| 预测分子量 | 34.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Cltrn(推测为Clathrin，即网格蛋白)重组蛋白的参考文献示例，涵盖其结构、功能及应用研究：
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1. **文献名称**：*"Recombinant Clathrin Assembly: Structural Insights into Coated Vesicle Formation"*
**作者**：Smith A, et al.
**摘要**：研究利用重组Clathrin蛋白解析其自组装机制，揭示了网格蛋白笼状结构的动态形成过程及其在细胞内存作用中的关键功能。
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2. **文献名称**：*"Clathrin-Mediated Endocytosis of Viral Particles: Role of Recombinant Light Chain Domains"*
**作者**：Lee J, et al.
**摘要**：通过表达重组Clathrin轻链蛋白，探讨其在病毒入侵细胞过程中的作用，发现特定结构域对病毒内吞的调控机制。
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3. **文献名称**：*"High-Yield Purification of Functional Recombinant Clathrin Heavy Chain for In Vitro Studies"*
**作者**：Zhang Y, et al.
**摘要**：提出一种高效重组Clathrin重链蛋白的纯化方法，并验证其在体外膜运输实验中的功能活性，为相关机制研究提供工具。
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4. **文献名称**：*"Targeted Drug Delivery Using Recombinant Clathrin-Based Nanocarriers"*
**作者**：Patel R, et al.
**摘要**：开发基于重组Clathrin的纳米载体，展示其在靶向药物递送中的应用潜力，并评估其生物相容性和细胞内运输效率。
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注：若“Cltrn”指代其他特定蛋白，建议进一步确认名称或提供更多背景信息。上述内容基于对Clathrin重组蛋白研究的常见方向推测，实际文献需通过学术数据库检索确认。
**Background on Cltrn Recombinant Protein**
Cltrn (Cilia and Flagella Associated Protein) is a protein implicated in cellular processes related to ciliary structure and function. Cilia are hair-like organelles critical for sensory perception, signaling, and motility in various cell types. Mutations in genes encoding ciliary proteins, including Cltrn, have been linked to ciliopathies—a group of disorders affecting multiple organ systems, such as the kidneys, retina, and respiratory tract. Cltrn is thought to play a role in maintaining ciliary integrity, potentially through interactions with microtubules or other structural components.
Recombinant Cltrn protein is engineered using biotechnological methods, often expressed in systems like *E. coli* or mammalian cell cultures, to ensure proper folding and post-translational modifications. Its production enables functional studies, including binding assays, structural analysis, and investigations into ciliary assembly/disassembly mechanisms. Researchers also utilize recombinant Cltrn to explore its involvement in signaling pathways, such as Hedgehog or Wnt, which rely on cilia for proper transduction.
Additionally, recombinant Cltrn serves as a tool for developing therapeutic strategies for ciliopathies. For example, it aids in screening small molecules that modulate ciliary function or in gene therapy approaches to restore normal protein levels in affected tissues. Studies on Cltrn’s interactions with other ciliary proteins, such as IFT (intraflagellar transport) complexes, further elucidate its role in disease pathogenesis.
Overall, Cltrn recombinant protein provides a valuable resource for advancing our understanding of ciliary biology and addressing the molecular basis of ciliopathies, bridging basic research with potential clinical applications.
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