| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | FIB1 |
| Uniprot No | Q9FEF8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-308aa |
| 氨基酸序列 | MRPPVTGGRGGGGFRGGRDGGGRGFGGGRSFGGGRSGDRGRSGPRGRGRGAPRGRGGPPRGGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKVEYRVWNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASFFLKTGGHFVISIKANCIDSTVAAEAVFQSEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKQKTPAS |
| 预测分子量 | 48.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FIB1重组蛋白的示例文献(非真实文献，仅作参考示例)：
1. **《重组FIB1蛋白在肿瘤转移中的功能研究》**
作者：Zhang L, et al.
摘要：本研究通过大肠杆菌系统表达并纯化FIB1重组蛋白，发现其通过抑制整合素信号通路显著降低肿瘤细胞的迁移和侵袭能力，提示FIB1可能作为潜在的抗转移靶点。
2. **《FIB1重组蛋白的晶体结构解析及功能验证》**
作者：Smith J, et al.
摘要：利用哺乳动物细胞表达系统获得高纯度FIB1蛋白，并通过X射线衍射解析其三维结构，揭示其与细胞外基质结合的活性位点，为设计靶向药物提供结构基础。
3. **《植物FIB1重组蛋白对纤维体形成的调控机制》**
作者：Wang Y, et al.
摘要：在拟南芥中表达重组FIB1蛋白，证实其参与细胞壁纤维体的动态组装，并通过基因敲除实验表明FIB1缺失导致植物细胞机械强度下降。
4. **《FIB1重组蛋白的免疫调节作用及其应用》**
作者：Kim S, et al.
摘要：研究发现，昆虫细胞表达的重组FIB1蛋白能够激活巨噬细胞的TLR4通路，增强先天性免疫应答，为开发新型免疫佐剂提供实验依据。
注：以上文献为模拟内容，实际研究中请根据具体领域和数据库(如PubMed、Web of Science)检索真实文献。
**Background of FIB1 Recombinant Protein**
FIB1 (Fibrillarin 1) is a highly conserved nucleolar protein involved in ribosomal RNA (rRNA) processing, particularly in the methylation and cleavage of precursor rRNA (pre-rRNA) during ribosome biogenesis. As a core component of small nucleolar ribonucleoproteins (snoRNPs), FIB1 plays a critical role in guiding site-specific 2′-O-methylation of ribosomal RNA through its association with box C/D small non-coding RNAs. This post-transcriptional modification is essential for ribosome assembly, stability, and function, thereby influencing cellular protein synthesis and growth.
Recombinant FIB1 protein is engineered using recombinant DNA technology, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its production enables detailed studies of ribosome biogenesis mechanisms, nucleolar organization, and interactions with other snoRNP components. Dysregulation of FIB1 has been implicated in diseases such as cancer, where aberrant ribosome biogenesis drives uncontrolled cell proliferation, and in autoimmune disorders like scleroderma, where autoantibodies target FIB1.
Researchers utilize recombinant FIB1 to investigate its role in stress responses, cell cycle regulation, and viral infections, as some pathogens exploit nucleolar functions for replication. Additionally, it serves as a tool for developing diagnostics or therapeutics targeting ribosome-related pathologies. Structural studies using recombinant FIB1 have revealed insights into its methyltransferase activity and RNA-binding domains, advancing understanding of epigenetic regulation and RNA metabolism. Challenges in production include maintaining solubility and enzymatic activity, often addressed through codon optimization or fusion tags. Overall, recombinant FIB1 remains pivotal in exploring fundamental cellular processes and disease mechanisms.
×
