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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGFL1 |
| Uniprot No | Q6UW32 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-110aa |
| 氨基酸序列 | APVAPMTPYLMLCQPHKRCGDKFYDPLQHCCYDDAVVPLARTQTCGNCTFRVCFEQCCPWTFMVKLINQNCDSARTSDDRLCRSVS |
| 预测分子量 | 38.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IGFL1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *IGFL1 promotes wound healing via activation of the EGFR/Akt/mTOR signaling pathway*
**作者**: Li Y, et al.
**摘要**: 研究通过重组人IGFL1蛋白处理皮肤细胞，发现其通过激活EGFR/Akt/mTOR通路促进细胞迁移和增殖，加速小鼠皮肤创伤修复。
2. **文献名称**: *Recombinant IGFL1 enhances intestinal epithelial regeneration in a colitis model*
**作者**: Zhang H, et al.
**摘要**: 利用大肠杆菌表达系统制备重组IGFL1蛋白，证明其通过上调Wnt/β-catenin通路促进肠上皮细胞再生，减轻小鼠结肠炎症状。
3. **文献名称**: *Structural and functional characterization of human IGFL1 protein*
**作者**: Wang X, et al.
**摘要**: 解析了哺乳动物细胞表达的重组IGFL1蛋白的晶体结构，发现其C端结构域对EGFR结合至关重要，并验证了其在肿瘤细胞侵袭中的促进作用。
注：以上文献为示例性质，实际研究中建议通过PubMed等数据库检索最新发表的具体论文。
IGFL1 (Insulin Growth Factor-Like Family Member 1) is a secreted protein encoded by the IGFL1 gene, part of the IGFL gene cluster located on human chromosome 19. Initially identified through genomic analysis, it shares structural similarities with insulin-like growth factors (IGFs) but exhibits distinct functional characteristics. The protein contains conserved cysteine-rich domains and a putative receptor-binding region, though its specific receptor and signaling mechanisms remain incompletely understood.
IGFL1 is expressed in various tissues, including skin, hair follicles, and certain epithelial cells, suggesting roles in tissue development and homeostasis. Studies implicate its involvement in cell proliferation, differentiation, and wound healing processes. Notably, IGFL1 expression is upregulated in some cancers, such as squamous cell carcinoma and breast cancer, correlating with tumor progression and metastasis, though its exact oncogenic mechanisms require further elucidation.
Recombinant IGFL1 protein is produced using expression systems (e.g., E. coli, mammalian cells) to enable functional studies. Purification typically involves affinity tags (e.g., His-tag) followed by refolding or chromatography to ensure bioactive conformation. This engineered protein serves as a critical tool for investigating IGFL1's interactions with potential receptors, downstream signaling pathways (e.g., MAPK/ERK, PI3K/AKT), and therapeutic applications. Emerging research explores its diagnostic potential as a biomarker and its modulation in regenerative medicine or anticancer therapies. Current challenges include clarifying its receptor specificity and resolving conflicting data regarding pro-tumorigenic versus tumor-suppressive roles across different cancer types.
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