| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Adck2 |
| Uniprot No | Q7Z695 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 128-389aa |
| 氨基酸序列 | TLWLHLLLKATETSGPTYIKLGQWASTRRDLFSEAFCAQFSKLHVRVTPHPWTHTERFLRQAFGDDWGSILSFENREPVGSGCVAQVYKAYANTAFLETDSVQRLGRASCLPPFSHTGAVGGLRELFGYLGNGRKPPENLADQSFLERLLLPKADLVGSNAGVSRAQVPGHQPEATNLISVAVKVLHPGLLAQVHMDLLLMKIGSRVLGVLPGIKWLSLPEIVEEFEKLMVQQIDLRYEAQNLEHFQVNFRNVKAVKFPTPL |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADCK2重组蛋白的3篇参考文献示例(注：文献信息为模拟示例，具体内容需根据实际论文调整)：
1. **文献名称**：*Functional characterization of recombinant ADCK2 kinase in mitochondrial CoQ biosynthesis*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化重组ADCK2蛋白，证实其参与辅酶Q(CoQ)生物合成的调控，通过体外激酶实验揭示其磷酸化特定底物的能力，为线粒体功能障碍相关疾病提供机制见解。
2. **文献名称**：*ADCK2 regulates cellular energy metabolism via interaction with electron transport chain components*
**作者**：Lee S, Kim M.
**摘要**：通过杆状病毒系统在昆虫细胞中表达重组ADCK2.发现其与线粒体复合物III结合，并调控ATP生成。研究利用重组蛋白进行互作实验，提示ADCK2在能量代谢中的关键作用。
3. **文献名称**：*Crystal structure and mutational analysis of the atypical kinase domain of ADCK2*
**作者**：Zhang R, et al.
**摘要**：解析了重组ADCK2激酶结构域的高分辨率晶体结构，发现其非典型激酶活性位点特征。通过点突变实验验证了关键氨基酸对酶活性的影响，为靶向药物设计奠定基础。
**注意事项**：实际文献需通过PubMed/Google Scholar检索关键词“ADCK2 recombinant”“ADCK2 kinase expression”确认。若研究较少，可扩展至ADCK家族(如ADCK3/4)或相关代谢通路文献。
**Background of ADCK2 Recombinant Protein**
ADCK2 (AarF domain-containing kinase 2) is a mitochondrial protein belonging to the ADCK family, which shares structural homology with yeast AarF kinases involved in coenzyme Q (CoQ) biosynthesis. Human ADCK2 is encoded by the *ADCK2* gene and plays a role in cellular energy metabolism, particularly in regulating CoQ-dependent pathways essential for mitochondrial electron transport and ATP production. Structurally, ADCK2 contains a kinase-like domain, though its catalytic activity remains debated, and a conserved ADCK-specific motif critical for interactions with CoQ-related enzymes.
Recombinant ADCK2 protein is engineered for in vitro studies to elucidate its molecular functions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant form often includes tags (e.g., His-tag) for purification and detection. Studies suggest ADCK2 interacts with mitochondrial complexes, influencing CoQ biosynthesis and lipid metabolism. Dysregulation of ADCK2 is linked to metabolic disorders, neurodegenerative diseases, and cancer, highlighting its therapeutic potential.
Research using recombinant ADCK2 focuses on characterizing its kinase activity, substrate specificity, and role in mitochondrial dynamics. It also aids in screening small molecules for modulating CoQ pathways or addressing mitochondrial dysfunction. Despite progress, questions remain about its precise enzymatic mechanism and regulatory networks, driving ongoing structural and functional investigations.
In summary, ADCK2 recombinant protein serves as a vital tool for decoding mitochondrial energy regulation and exploring therapeutic strategies for diseases tied to metabolic and oxidative stress pathways.
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