| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TWF2 |
| Uniprot No | Q6IBS0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-349aa |
| 氨基酸序列 | AHQTGIHATEELKEFFAKARAGSVRLIKVVIEDEQLVLGASQEPVGRWDQDYDRAVLPLLDAQQPCYLLYRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDELFGTVKDDLSFAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLAFPLQPEAQRALQQLKQKMVNYIQMKLDLERETIELVHTEPTDVAQLPSRVPRDAARYHFFLYKHTHEGDPLESVVFIYSMPGYKCSIKERMLYSSCKSRLLDSVEQDFHLEIAKKIEIGDGAELTAEFLYDEVHPKQHAFKQAFAKPKGPGGKRGHKRLIRGPGENGDDS |
| 预测分子量 | 66.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TWF2(Twinfilin-2)重组蛋白的3篇参考文献(内容基于模拟学术文献框架，供参考)：
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1. **文献名称**: *"Recombinant human Twinfilin-2 expression, purification, and functional characterization in actin dynamics"*
**作者**: Smith J., et al.
**摘要**: 该研究报道了人源TWF2重组蛋白在大肠杆菌中的高效表达与纯化方法，验证了其通过结合肌动蛋白单体(G-actin)抑制丝状肌动蛋白(F-actin)组装的功能，并揭示了其磷酸化修饰对细胞迁移的影响。
2. **文献名称**: *"Structural insights into the actin-binding domains of Twinfilin-2 through recombinant protein crystallography"*
**作者**: Tanaka K., et al.
**摘要**: 通过X射线晶体学解析了重组TWF2蛋白的N端和C端肌动蛋白结合域的三维结构，阐明了其通过双肌动蛋白结合域协同调控细胞骨架重塑的分子机制。
3. **文献名称**: *"Twinfilin-2 recombinant protein suppresses breast cancer metastasis by targeting invadopodia formation"*
**作者**: Chen L., et al.
**摘要**: 研究发现重组TWF2蛋白能通过竞争性结合游离肌动蛋白，抑制乳腺癌细胞侵袭伪足(invadopodia)的形成，从而降低癌细胞体外侵袭和体内转移能力。
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**注**：以上文献为示例性内容，实际研究中请以真实发表的论文为准。建议通过PubMed或Web of Science搜索关键词“Twinfilin-2 recombinant”获取具体文献。
**Background of TWF2 Recombinant Protein**
Twinfilin-2 (TWF2) is a member of the actin-binding protein family, primarily involved in regulating cytoskeletal dynamics by interacting with actin monomers and filaments. It plays a critical role in cellular processes such as cell migration, endocytosis, and cell division. Structurally, TWF2 contains two actin-depolymerizing factor homology (ADF-H) domains, which enable its binding to actin, and a C-terminal extension that mediates interactions with other regulatory proteins. Dysregulation of TWF2 has been implicated in pathological conditions, including cancer metastasis and neurodegenerative disorders, highlighting its importance in cellular homeostasis.
Recombinant TWF2 protein is engineered using genetic engineering techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. The recombinant form allows researchers to study TWF2’s biochemical properties, interactions with binding partners (e.g., actin, capping proteins), and its regulatory mechanisms in vitro. Its production facilitates functional assays, such as actin polymerization/depolymerization kinetics, and enables the exploration of TWF2’s role in signaling pathways linked to cytoskeletal remodeling.
Studies using recombinant TWF2 have advanced understanding of how spatial and temporal control of actin dynamics influences cell motility and morphology. Additionally, TWF2 has emerged as a potential therapeutic target; inhibitors or modulators of its activity could offer strategies to disrupt pathological processes like tumor invasion. As a research tool, recombinant TWF2 is invaluable for structural studies (e.g., crystallography), antibody development, and high-throughput screening for drug discovery. Its application extends to modeling diseases in vitro and identifying biomarkers associated with cytoskeletal dysfunction.
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