| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | isdA |
| Uniprot No | Q6GA85 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 47-316aa |
| 氨基酸序列 | ATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYYFQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIVVPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEKVKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVATAKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKT |
| 预测分子量 | 35.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于isdA重组蛋白的3篇参考文献示例(文献信息为虚构示例，仅作格式参考)：
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1. **文献名称**: *IsdA-mediated iron acquisition in Staphylococcus aureus*
**作者**: Torres, V.J., et al.
**摘要**: 研究揭示了金黄色葡萄球菌isdA基因编码的表面蛋白IsdA在铁摄取中的作用。通过重组表达IsdA蛋白，发现其能结合宿主血红素，并参与细菌黏附和免疫逃逸机制。
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2. **文献名称**: *Structural and functional analysis of recombinant IsdA protein*
**作者**: Grigg, J.C., Murphy, M.E.P.
**摘要**: 通过大肠杆菌表达系统重组生成IsdA蛋白，解析其晶体结构，证明其通过保守结构域与血红素结合，为开发针对金黄色葡萄球菌的抑制剂提供依据。
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3. **文献名称**: *Recombinant IsdA as a vaccine candidate against S. aureus*
**作者**: Dryla, A., et al.
**摘要**: 评估重组IsdA蛋白的免疫原性，动物实验表明其能诱导保护性抗体，显著降低感染模型中的细菌载量，提示其作为疫苗靶点的潜力。
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*注：以上文献为模拟示例，实际引用需查询PubMed、Web of Science等数据库获取真实数据。建议结合关键词“isdA recombinant protein”或“Staphylococcus aureus IsdA”进行检索。*
The isdA recombinant protein originates from the *Staphylococcus aureus* genome, specifically from the iron-regulated surface determinant (Isd) system. This system enables the bacterium to scavenge heme iron from host hemoglobin during infection, a critical adaptation for survival in iron-limited host environments. The *isdA* gene encodes a surface-exposed protein that binds hemoglobin and facilitates heme extraction, playing a key role in bacterial virulence and iron acquisition.
Recombinant isdA protein is produced through genetic engineering, typically by cloning the *isdA* gene into expression vectors (e.g., in *E. coli*), followed by purification using affinity chromatography. Its recombinant form retains functional domains responsible for hemoglobin binding, making it a valuable tool for studying *S. aureus* pathogenesis, host-pathogen interactions, and iron metabolism. Researchers also explore its potential in vaccine development, as antibodies targeting IsdA could neutralize bacterial iron uptake and reduce infectivity.
Beyond basic research, isdA recombinant protein has applications in diagnostic assays and therapeutic antibody production. However, challenges remain in ensuring proper protein folding and post-translational modifications in heterologous systems, which may affect its antigenicity or functional mimicry of the native protein. Ongoing studies aim to optimize expression conditions and evaluate its efficacy in preclinical models as a prophylactic or therapeutic target against antibiotic-resistant *S. aureus* strains.
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