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Recombinant Mouse Plbd2 protein

  • 中文名: 推定型磷脂酶B样2(Plbd2)重组蛋白
  • 别    名: Plbd2;Putative phospholipase B-like 2
货号: PA2000-3086
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Mouse
靶点Plbd2
Uniprot No Q3TCN2
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间 47-594aa
氨基酸序列LPTLGPGWQRQNPDPPVSRTRSLLLDAASGQLRLEDGFHPDAVAWANLTNAIRETGWAYLDLSTNGRYNDSLQAYAAGVVEASVSEELIYMHWMNTVVNYCGPFEYEVGYCEKLKNFLEANLEWMQREMELNPDSPYWHQVRLTLLQLKGLEDSYEGRLTFPTGRFTIKPLGFLLLQISGDLEDLEPALNKTNTKPSLGSGSCSALIKLLPGGHDLLVAHNTWNSYQNMLRIIKKYRLQFREGPQEEYPLVAGNNLVFSSYPGTIFSGDDFYILGSGLVTLETTIGNKNPALWKYVQPQGCVLEWIRNVVANRLALDGATWADVFKRFNSGTYNNQWMIVDYKAFLPNGPSPGSRVLTILEQIPGMVVVADKTAELYKTTYWASYNIPYFETVFNASGLQALVAQYGDWFSYTKNPRAKIFQRDQSLVEDMDAMVRLMRYNDFLHDPLSLCEACNPKPNAENAISARSDLNPANGSYPFQALHQRAHGGIDVKVTSFTLAKYMSMLAASGPTWDQCPPFQWSKSPFHSMLHMGQPDLWMFSPIRVPWD
预测分子量 62.7 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PLBD2重组蛋白的模拟参考文献示例(注:以下信息为假设性示例,实际文献需通过学术数据库核实):

1. **文献名称**:*Expression and Purification of Recombinant PLBD2 in E. coli for Functional Characterization*

**作者**:Zhang Y, et al. (2020)

**摘要**:报道了在大肠杆菌中成功表达并纯化PLBD2重组蛋白,优化了表达条件,证实其具有磷脂酶活性,并分析了其在脂质代谢中的潜在作用。

2. **文献名称**:*PLBD2 Promotes Tumor Angiogenesis via Regulation of Lipid Signaling in Colorectal Cancer*

**作者**:Li H, et al. (2018)

**摘要**:利用重组PLBD2蛋白进行体外实验,发现其通过调控溶血磷脂酸(LPA)信号通路促进结直肠癌细胞血管生成,提示其作为癌症治疗靶点的可能性。

3. **文献名称**:*Crystal Structure of Human PLBD2 Reveals a Unique Catalytic Domain Organization*

**作者**:Smith J, et al. (2021)

**摘要**:通过X射线晶体学解析了PLBD2重组蛋白的三维结构,揭示了其催化结构域的特殊构象,为设计特异性抑制剂提供了结构基础。

4. **文献名称**:*Recombinant PLBD2 Modulates Macrophage Polarization in Inflammatory Diseases*

**作者**:Chen L, et al. (2019)

**摘要**:研究表明,重组PLBD2蛋白通过调节巨噬细胞极化影响炎症反应,在动物模型中减轻了实验性关节炎症状。

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建议通过PubMed或Web of Science以“PLBD2 recombinant protein”、“PLBD2 expression”等关键词检索真实文献。

背景信息

**Background of PLBD2 Recombinant Protein**

Phospholipase B domain-containing protein 2 (PLBD2) is a conserved eukaryotic protein implicated in lipid metabolism and membrane remodeling processes. It belongs to the phospholipase B-like family, characterized by a conserved catalytic domain with potential hydrolase activity, though its exact enzymatic function remains under investigation. PLBD2 is expressed in various tissues, with higher levels observed in immune cells, suggesting a role in inflammation or immune regulation. Studies associate PLBD2 with lipid droplet dynamics, lysosomal function, and cellular signaling pathways, potentially influencing processes like autophagy and apoptosis.

Recombinant PLBD2 protein is engineered for *in vitro* studies to elucidate its biochemical properties and interactions. Produced using prokaryotic (e.g., *E. coli*) or eukaryotic (e.g., mammalian or insect cell) expression systems, the recombinant form often includes tags (e.g., His-tag) for purification and detection. Its production enables structural analysis (e.g., X-ray crystallography, cryo-EM) and functional assays to explore substrate specificity, enzymatic activity, or binding partners.

Emerging evidence links PLBD2 to human diseases. Elevated PLBD2 levels are reported in certain cancers, metabolic disorders, and neurodegenerative conditions, hinting at its potential as a biomarker or therapeutic target. Recombinant PLBD2 also aids in antibody development for diagnostic applications. However, mechanistic insights into its physiological and pathological roles remain limited, driving ongoing research to decode its contributions to cellular homeostasis and disease progression.

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