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Recombinant E.coli LCY1 protein

  • 中文名: 番茄红素-环化酶,叶绿体(LCY1)重组蛋白
  • 别    名: LCY1;CRTL;Lycopene beta cyclase, chloroplastic/chromoplastic
货号: PA2000-3079
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属E.coli 
靶点LCY1
Uniprot No Q38933
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间 81-501aa
氨基酸序列QVVDLAIVGGGPAGLAVAQQVSEAGLSVCSIDPSPKLIWPNNYGVWVDEFEAMDLLDCLDTTWSGAVVYVDEGVKKDLSRPYGRVNRKQLKSKMLQKCITNGVKFHQSKVTNVVHEEANSTVVCSDGVKIQASVVLDATGFSRCLVQYDKPYNPGYQVAYGIVAEVDGHPFDVDKMVFMDWRDKHLDSYPELKERNSKIPTFLYAMPFSSNRIFLEETSLVARPGLRMEDIQERMAARLKHLGINVKRIEEDERCVIPMGGPLPVLPQRVVGIGGTAGMVHPSTGYMVARTLAAAPIVANAIVRYLGSPSSNSLRGDQLSAEVWRDLWPIERRRQREFFCFGMDILLKLDLDATRRFFDAFFDLQPHYWHGFLSSRLFLPELLVFGLSLFSHASNTSRLEIMTKGTVPLAKMINNLVQDRD
预测分子量 49.1 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于LCY1重组蛋白的模拟参考文献示例(仅供参考,实际文献需通过学术数据库查询):

1. **文献名称**:*Cloning and Functional Characterization of Recombinant LCY1 from Solanum lycopersicum*

**作者**:Zhang Y, et al.

**摘要**:本研究克隆了番茄(Solanum lycopersicum)中的番茄红素环化酶1(LCY1)基因,并在大肠杆菌中成功表达重组蛋白。通过体外酶活性实验证实重组LCY1能够催化番茄红素向β-胡萝卜素的转化,为类胡萝卜素合成机制研究提供基础。

2. **文献名称**:*Heterologous Expression of LCY1 in Arabidopsis Thaliana Enhances Carotenoid Accumulation*

**作者**:Wang H, et al.

**摘要**:作者通过构建LCY1重组表达载体并转化拟南芥,发现过表达LCY1显著提高了植物叶片中β-胡萝卜素和叶黄素的含量,表明该酶在类胡萝卜素代谢工程中具有应用潜力。

3. **文献名称**:*Structural Analysis of Recombinant LCY1 Reveals Key Catalytic Domains*

**作者**:Li X, et al.

**摘要**:通过X射线晶体学解析了重组LCY1的三维结构,鉴定出其活性位点和底物结合区域,为理解该酶的催化机制及设计功能突变体提供了结构基础。

4. **文献名称**:*Optimization of LCY1 Recombinant Protein Production in Pichia pastoris*

**作者**:Chen J, et al.

**摘要**:研究在毕赤酵母系统中优化LCY1重组蛋白的表达条件,通过发酵工艺改进使蛋白产量提高3倍,并验证了其体外催化活性,为规模化生产奠定基础。

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**备注**:以上为示例文献,实际研究中建议通过 **PubMed**、**Web of Science** 或 **Google Scholar** 检索关键词 "LCY1 recombinant protein" 或 "lycopene cyclase 1 heterologous expression" 获取真实文献。

背景信息

**Background of LCY1 Recombinant Protein**

LCY1 (Lycopene Cyclase 1) is a key enzyme in the carotenoid biosynthesis pathway, primarily responsible for catalyzing the cyclization of lycopene into β-carotene, a precursor for essential metabolites like vitamin A and plant hormones. This enzyme is critical in plants, algae, and certain microorganisms, influencing pigmentation, photosynthesis, and stress responses. Its role in converting linear lycopene into cyclic carotenoids also contributes to antioxidant properties, which are vital for protecting organisms from oxidative damage.

The recombinant LCY1 protein is produced through genetic engineering, where the *LCY1* gene is cloned into expression vectors (e.g., *E. coli* or yeast systems) to enable large-scale protein synthesis. Recombinant technology allows for precise control over protein purity and functionality, bypassing challenges associated with extracting LCY1 from natural sources. Studies on LCY1 have expanded due to its biotechnological potential, including enhancing nutritional profiles in crops (e.g., Golden Rice), improving stress tolerance in plants, and exploring therapeutic applications linked to vitamin A deficiency or oxidative stress-related diseases.

Recent research also focuses on LCY1's structural and mechanistic insights, aiming to engineer variants with higher catalytic efficiency or substrate specificity. Challenges remain in optimizing expression systems and ensuring stability, but advancements in protein engineering and synthetic biology continue to drive progress. Overall, LCY1 recombinant protein serves as a valuable tool in both agricultural and biomedical fields, bridging gaps between metabolic engineering and human health.

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