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| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Ang4 |
| Uniprot No | Q3TMQ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-144aa |
| 氨基酸序列 | QNERYEKFLRQHYDAKPNGRDDRYCESMMKERKLTSPCKDVNTFIHGTKKNIRAICGKKGSPYGENFRISNSPFQITTCTHSGASPRPPCGYRAFKDFRYIVIACEDGWPVHFDESFISP |
| 预测分子量 | 29.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Ang4重组蛋白的参考文献示例(注：以下内容为示例，实际文献需根据具体研究核实)：
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1. **标题**: *"Angiogenin-4 expression in Paneth cells regulates gut microbiota composition"*
**作者**: Hooper LV, et al.
**摘要**: 研究报道了重组Ang4蛋白在肠道潘氏细胞中的表达，证实其通过抗菌活性调控肠道菌群平衡，影响宿主防御机制。
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2. **标题**: *"Structural insights into the enzymatic mechanism of recombinant Ang4"*
**作者**: Shi Y, et al.
**摘要**: 通过X射线晶体学解析重组Ang4的三维结构，揭示了其RNA酶活性位点及与底物结合的关键区域，为功能研究提供结构基础。
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3. **标题**: *"Recombinant Ang4 mitigates colitis via modulating mucosal immunity"*
**作者**: Kumar P, et al.
**摘要**: 实验表明，重组Ang4蛋白通过抑制致病菌生长并促进保护性细胞因子分泌，缓解小鼠结肠炎模型中的肠道炎症。
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4. **标题**: *"Antimicrobial activity of recombinant Ang4 against Gram-negative pathogens"*
**作者**: Smith AB, et al.
**摘要**: 体外实验证实重组Ang4对大肠杆菌等革兰氏阴性菌具有选择性杀伤作用，机制涉及破坏细菌膜完整性。
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**提示**：以上为模拟参考文献，实际研究中请通过PubMed、Google Scholar等平台以“Angiogenin-4”、“recombinant Ang4”等关键词检索最新文献。
Angiopoietin-4 (Ang4), a member of the angiopoietin family, is a secreted glycoprotein critically involved in vascular development and remodeling. Initially identified in the early 2000s, Ang4 shares structural homology with other angiopoietins (Ang1. Ang2. Ang3), characterized by a coiled-coil domain and a fibrinogen-like domain. It primarily binds to the Tie2 receptor tyrosine kinase, modulating endothelial cell signaling. While Ang1 promotes vascular stabilization and maturation, Ang4 exhibits context-dependent roles, influencing both angiogenesis and vascular permeability. Its expression is notably enriched in tissues such as the lung, placenta, and gastrointestinal tract, with emerging links to mucosal immunity and gut microbiota interactions.
Recombinant Ang4 (rAng4) is engineered using mammalian or bacterial expression systems, enabling studies on its biochemical and functional properties. Purification typically involves affinity chromatography, ensuring high specificity and activity. Research highlights Ang4's dual role in vascular biology: it may stabilize blood vessels under physiological conditions but contribute to pathological angiogenesis in diseases like cancer or inflammatory bowel disease (IBD). For instance, in murine models, Ang4 overexpression correlates with enhanced intestinal vascular integrity and reduced inflammation, suggesting therapeutic potential for IBD. Conversely, in tumor microenvironments, Ang4 may synergize with VEGF to fuel aberrant vasculature, prompting exploration of Ang4 inhibitors in oncology.
Current studies also investigate Ang4's interplay with microbial metabolites, as gut microbiota dysregulation alters Ang4 expression, impacting mucosal barrier function. Despite progress, Ang4's signaling mechanisms remain less defined compared to Ang1/Ang2. partly due to species-specific differences (e.g., murine Ang3 is a human Ang4 homolog). Challenges include elucidating its pleiotropic effects and optimizing rAng4 for clinical use. Ongoing research aims to decode its role in tissue repair, immune modulation, and metabolic diseases, positioning Ang4 as a multifaceted target in precision medicine.
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