首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADIPOQ |
| Uniprot No | Q15848 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-244aa |
| 氨基酸序列 | MLLLGAVLLLLALPGHDQETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN |
| 预测分子量 | 26,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADIPOQ重组蛋白的3篇代表性文献摘要(模拟示例，非真实文献)：
---
1. **文献名称**: "Production and functional characterization of recombinant human adiponectin (ADIPOQ) in Escherichia coli"
**作者**: Smith A, et al.
**摘要**: 本研究描述了一种在大肠杆菌中高效表达和纯化重组人ADIPOQ蛋白的方法，并通过体外实验验证其活性。结果显示，该重组蛋白能有效激活AMPK信号通路，增强脂肪细胞的胰岛素敏感性。
---
2. **文献名称**: "Recombinant adiponectin ameliorates insulin resistance in diabetic mouse models via adipose tissue modulation"
**作者**: Tanaka Y, et al.
**摘要**: 通过动物实验验证重组ADIPOQ蛋白对2型糖尿病模型的治疗潜力。研究发现，注射重组ADIPOQ显著降低血糖水平，改善脂代谢紊乱，并减少脂肪组织炎症反应。
---
3. **文献名称**: "Structural insights into the oligomerization of ADIPOQ recombinant protein and its receptor binding mechanism"
**作者**: Lee H, et al.
**摘要**: 利用X射线晶体学解析了重组ADIPOQ蛋白的高阶寡聚结构，揭示了其与AdipoR1/2受体结合的关键结构域，为开发基于脂联素的代谢疾病治疗策略提供分子基础。
---
4. **文献名称**: "Recombinant adiponectin attenuates hepatic steatosis in metabolic syndrome through PPAR-α activation"
**作者**: Zhang R, et al.
**摘要**: 研究证明重组ADIPOQ蛋白通过激活PPAR-α通路，显著减少高脂饮食诱导的小鼠肝脏脂肪沉积，提示其在非酒精性脂肪肝治疗中的应用前景。
---
注：以上内容为模拟生成，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
**Background of ADIPOQ Recombinant Protein**
ADIPOQ, also known as adiponectin, is a hormone predominantly secreted by adipose tissue, playing a critical role in regulating glucose metabolism, fatty acid oxidation, and insulin sensitivity. It circulates in the bloodstream as multiple oligomeric complexes, including low-molecular-weight trimers, mid-sized hexamers, and high-molecular-weight (HMW) multimers, each with distinct biological activities. ADIPOQ binds to adiponectin receptors (AdipoR1 and AdipoR2), activating downstream pathways like AMPK and PPAR-α, which modulate energy homeostasis and anti-inflammatory responses.
Reduced plasma adiponectin levels are strongly associated with metabolic disorders such as obesity, type 2 diabetes, and cardiovascular diseases. This correlation has driven interest in recombinant ADIPOQ protein as a potential therapeutic agent or research tool. Recombinant ADIPOQ is produced using expression systems like *E. coli* or mammalian cells, followed by purification to ensure bioactivity. Its applications span *in vitro* and *in vivo* studies to elucidate metabolic pathways, evaluate drug candidates, or explore replacement therapies.
Researchers also utilize ADIPOQ recombinant protein to investigate its structural-functional relationships, particularly the role of post-translational modifications (e.g., hydroxylation and glycosylation) in receptor binding and signaling. Despite challenges in maintaining the native multimeric conformation during production, advances in protein engineering have improved the stability and efficacy of recombinant forms.
Overall, ADIPOQ recombinant protein serves as a vital resource for understanding metabolic regulation and developing interventions targeting obesity-related diseases. Its therapeutic potential, combined with ongoing research into isoform-specific effects, continues to make it a focal point in metabolic and endocrine studies.
×
