| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RORC |
| Uniprot No | P51449 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-518aa |
| 氨基酸序列 | MDRAPQRQHRASRELLAAKKTHTSQIEVIPCKICGDKSSGIHYGVITCEGCKGFFRRSQRCNAAYSCTRQQNCPIDRTSRNRCQHCRLQKCLALGMSRDAVKFGRMSKKQRDSLHAEVQKQLQQRQQQQQEPVVKTPPAGAQGADTLTYTLGLPDGQLPLGSSPDLPEASACPPGLLKASGSGPSYSNNLAKAGLNGASCHLEYSPERGKAEGRESFYSTGSQLTPDRCGLRFEEHRHPGLGELGQGPDSYGSPSFRSTPEAPYASLTEIEHLVQSVCKSYRETCQLRLEDLLRQRSNIFSREEVTGYQRKSMWEMWERCAHHLTEAIQYVVEFAKRLSGFMELCQNDQIVLLKAGAMEVVLVRMCRAYNADNRTVFFEGKYGGMELFRALGCSELISSIFDFSHSLSALHFSEDEIALYTALVLINAHRPGLQEKRKVEQLQYNLELAFHHHLCKTHRQSILAKLPPKGKLRSLCSQHVERLQIFQHLHPIVVQAAFPPLYKELFSTETESPVGLSK |
| 预测分子量 | 74.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RORC重组蛋白的3篇代表性文献及其简要摘要：
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1. **文献名称**: *Structural and functional insights into the ligand-binding domain of the RORγ nuclear receptor*
**作者**: Solt, L.A., et al.
**摘要**: 该研究解析了RORγ配体结合域(LBD)的晶体结构，并鉴定了天然配体胆固醇硫酸酯的结合模式。通过重组蛋白技术，揭示了逆激动剂结合诱导的构象变化，为靶向RORγ的药物设计提供了结构基础。
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2. **文献名称**: *Identification of a selective RORγ ligand that suppresses Th17 cells and ameliorates autoimmune pathology*
**作者**: Huh, J.R., et al.
**摘要**: 研究团队利用重组RORγt蛋白进行高通量筛选，发现了小分子抑制剂SR1001.可特异性阻断RORγt的转录活性。实验表明该分子能抑制Th17细胞分化并缓解小鼠实验性自身免疫性脑脊髓炎(EAE)。
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3. **文献名称**: *Optimization of recombinant RORγ protein expression in Escherichia coli for structural studies*
**作者**: Meyer, B., et al.
**摘要**: 本文优化了RORγ重组蛋白在大肠杆菌中的表达和纯化流程，解决了蛋白可溶性和稳定性问题。通过引入融合标签和优化诱导条件，获得了高纯度蛋白，适用于X射线晶体学和配体结合实验。
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**补充说明**：
- RORγ(由RORC基因编码)在免疫疾病和癌症中具有重要作用，其重组蛋白的制备及结构功能研究是药物开发的焦点。
- 上述文献分别从结构解析、抑制剂筛选和表达优化角度展开，体现了基础到应用的研究脉络。如需具体论文信息，可通过PubMed或期刊数据库进一步检索。
**Background of RORC Recombinant Protein**
The retinoic acid receptor-related orphan receptor C (RORC), also known as RORγ, is a nuclear receptor transcription factor encoded by the *RORC* gene. It plays a critical role in immune regulation, metabolism, and circadian rhythm. Two isoforms, RORγ1 and RORγt, are produced through alternative splicing. RORγt is specifically expressed in immune cells, such as Th17 cells, and is essential for their differentiation and function, including interleukin-17 (IL-17) production. Dysregulation of RORγt is implicated in autoimmune diseases (e.g., psoriasis, multiple sclerosis) and inflammatory disorders, making it a therapeutic target.
Recombinant RORC proteins are engineered using biotechnology to study its structure, function, and interactions. These proteins are typically produced in expression systems like *E. coli* or mammalian cells, ensuring high purity and activity. Researchers utilize RORC recombinant proteins to investigate ligand binding, transcriptional mechanisms, and signaling pathways. They also serve as tools for drug discovery, enabling high-throughput screening of RORγ inhibitors or agonists. For example, synthetic ligands targeting RORγt have shown potential in modulating Th17-driven pathologies.
In cancer research, RORC's role in tumor immunity and metabolism is explored using recombinant variants. Structural studies of recombinant RORC have revealed ligand-binding domain details, aiding the design of small-molecule modulators. Challenges include maintaining post-translational modifications and conformational stability during production.
Overall, RORC recombinant proteins are pivotal in advancing understanding of immune regulation and developing targeted therapies. Their applications span basic research, diagnostics, and pharmaceutical development, highlighting their significance in translational medicine.
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