| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REL |
| Uniprot No | Q04864 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 3-616aa |
| 氨基酸序列 | SGAYNPYIEIIEQPRQRGMRFRYKCEGRSAGSIPGEHSTDNNRTYPSIQIMNYYGKGKVRITLVTKNDPYKPHPHDLVGKDCRDGYYEAEFGQERRPLFFQNLGIRCVKKKEVKEAIITRIKAGINPFNVPEKQLNDIEDCDLNVVRLCFQVFLPDEHGNLTTALPPVVSNPIYDNRAPNTAELRICRVNKNCGSVRGGDEIFLLCDKVQKDDIEVRFVLNDWEAKGIFSQADVHRQVAIVFKTPPYCKAITEPVTVKMQLRRPSDQEVSESMDFRYLPDEKDTYGNKAKKQKTTLLFQKLCQDHVETGFRHVDQDGLELLTSGDPPTLASQSAGITVNFPERPRPGLLGSIGEGRYFKKEPNLFSHDAVVREMPTGVSSQAESYYPSPGPISSGLSHHASMAPLPSSSWSSVAHPTPRSGNTNPLSSFSTRTLPSNSQGIPPFLRIPVGNDLNASNACIYNNADDIVGMEASSMPSADLYGISDPNMLSNCSVNMMTTSSDSMGETDNPRLLSMNLENPSCNSVLDPRDLRQLHQMSSSSMSAGANSNTTVFVSQSDAFEGSDFSCADNSMINESGPSNSTNPNSHGFVQDSQYSGIGSMQNEQLSDSFPYEF |
| 预测分子量 | 71.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于REL重组蛋白的3篇参考文献示例(内容为模拟生成，非真实文献)：
1. **文献名称**：*Expression and Purification of Recombinant REL Protein for NF-κB Pathway Studies*
**作者**：Smith J, et al.
**摘要**：本研究描述了大肠杆菌中重组REL蛋白的高效表达与纯化方法，验证了其与NF-κB DNA结合位点的相互作用，并用于体外信号通路功能分析。
2. **文献名称**：*Structural Insights into the Dimerization of Recombinant Human REL*
**作者**：Lee C, et al.
**摘要**：通过X射线晶体学解析了重组人源REL蛋白的二聚化结构，揭示了其C端反式激活结构域的关键功能位点，为靶向药物设计提供了基础。
3. **文献名称**：*Recombinant REL as a Tool for Screening Inhibitors in Chronic Inflammation*
**作者**：Garcia R, et al.
**摘要**：利用昆虫细胞表达系统制备功能性重组REL蛋白，建立高通量筛选平台，鉴定出多个抑制REL介导炎症信号的小分子化合物。
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如需真实文献，建议通过PubMed或Web of Science检索关键词(如"Recombinant REL protein" "NFKB3 expression")，或参考《Journal of Biological Chemistry》《Cell Signaling》等期刊的相关研究。
REL recombinant proteins are derived from the REL family of transcription factors, key components of the NF-κB signaling pathway. The REL family, including c-REL, RELA (p65), and RELB, regulates genes involved in immune responses, inflammation, cell survival, and proliferation. c-REL, encoded by the *REL* gene, is particularly notable for its role in lymphocyte activation and immune regulation. Dysregulation of REL proteins is linked to cancers, autoimmune diseases, and chronic inflammation, underscoring their biomedical significance.
Recombinant REL proteins are engineered using genetic cloning techniques, where the *REL* gene is expressed in host systems (e.g., *E. coli*, mammalian cells) to produce purified, functional proteins. These proteins retain critical domains, such as the N-terminal Rel homology domain (RHD), responsible for DNA binding and dimerization, and the C-terminal transactivation domain. Researchers often generate mutant variants (e.g., phosphorylation-deficient or constitutively active forms) to study signaling mechanisms or disease-related dysfunctions.
Applications of REL recombinant proteins include *in vitro* studies of NF-κB pathway dynamics, protein-DNA/ protein-protein interactions, and high-throughput drug screening for therapies targeting inflammatory or oncogenic pathways. Their use in structural biology (e.g., crystallography) has elucidated mechanisms of DNA binding and inhibitor interactions. Additionally, recombinant REL serves as a tool for developing diagnostic assays or gene therapy strategies aimed at modulating NF-κB activity.
Despite challenges like protein stability and post-translational modification mimicry, recombinant REL proteins remain indispensable for dissecting NF-κB biology and advancing therapeutic innovations. Their versatility continues to drive insights into immune regulation and disease pathogenesis.
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