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Recombinant Human ROPN1B protein

  • 中文名: Rhophilin相关蛋白1B(ROPN1B)重组蛋白
  • 别    名: ROPN1B;Ropporin-1B
货号: PA2000-2186
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数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ROPN1B
Uniprot No Q9BZX4
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间 1-120aa
氨基酸序列MWKVVNLPTDLFNSVMNVGRFTEEIEWLKFLALACSALGVTITKTLKIVCEVLSCDHNGGLPRIPFSTFQFLYTYIAEVDGEICASHVSRMLNYIEQEVIGPDGLITVNDFTQNPRVWLE
预测分子量 15.6 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ROPN1B重组蛋白的3篇参考文献示例(注:内容基于模拟生成,非真实文献):

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1. **文献名称**: Recombinant ROPN1B Expression in E. coli and Its Role in Sperm Motility

**作者**: Zhang Y, et al. (2015)

**摘要**: 本研究成功在大肠杆菌中表达并纯化重组ROPN1B蛋白,证实其与精子鞭毛蛋白相互作用,体外实验表明ROPN1B缺失会显著降低精子运动能力,提示其在男性生殖功能中的关键作用。

2. **文献名称**: Structural and Functional Analysis of ROPN1B in Cancer Cell Signaling

**作者**: Chen L, et al. (2018)

**摘要**: 通过哺乳动物细胞系统表达带His标签的ROPN1B重组蛋白,结合质谱分析发现其通过调控RhoA/ROCK通路促进癌细胞迁移,为靶向ROPN1B的肿瘤治疗提供理论基础。

3. **文献名称**: Crystallographic Study of ROPN1B Reveals Potential Drug-Binding Sites

**作者**: Liu X, et al. (2020)

**摘要**: 利用X射线晶体学解析ROPN1B重组蛋白的三维结构,发现其激酶结构域中的特异性口袋,为开发针对男性不育或癌症的小分子抑制剂奠定结构基础。

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以上内容为模拟生成,实际文献需通过PubMed、Google Scholar等平台检索确认。

背景信息

**Background of ROPN1B Recombinant Protein**

ROPN1B (Rhophilin-associated tail protein 1B), also known as Ropporin-1B, is a member of the Ropporin protein family, primarily expressed in sperm cells and associated with reproductive biology. It plays a critical role in sperm motility and fertilization by interacting with Rhophilin-1. a protein involved in regulating cytoskeletal dynamics. Structurally, ROPN1B contains conserved domains, including an N-terminal coiled-coil region and a C-terminal RII-binding domain, facilitating its interaction with regulatory subunits of protein kinase A (PKA) and other signaling molecules.

The protein is localized to the fibrous sheath of sperm flagella, where it contributes to the structural integrity and functional coordination of the tail apparatus. Studies suggest ROPN1B is essential for sperm movement by modulating PKA-mediated signaling pathways, which regulate phosphorylation events critical for flagellar bending and hyperactivation. Dysregulation of ROPN1B has been linked to male infertility, highlighting its clinical relevance.

Recombinant ROPN1B is produced using expression systems like *E. coli* or mammalian cells, enabling in vitro studies of its biochemical properties, interaction networks, and role in spermatogenesis. Its recombinant form is widely utilized in fertility research, drug screening, and diagnostic assay development to explore molecular mechanisms underlying sperm dysfunction. Additionally, ROPN1B serves as a biomarker candidate for assessing male reproductive health. Recent advances in structural biology and gene-editing tools, such as CRISPR, have further expanded its applications in studying fertilization-related disorders and potential therapeutic interventions.

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