| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF212 |
| Uniprot No | Q495C1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-297aa |
| 氨基酸序列 | MANWVFCNRCFQPPHRTSCFSLTNCGHVYCDACLGKGKKNECLICKAPCRTVLLSKHTDADIQAFFMSIDSLCKKYSRETSQILEFQEKHRKRLLAFYREKISRLEESLRKSVLQIEQLQSMRSSQQTAFSTIKSSVSTKPHGCLLPPHSSAPDRLESMEVDLSPSPIRKSEIAAGPARISMISPPQDGRMGPHLTASFCFIPWLTLSKPPVPGECVISRGSPCFCIDVCPHWLLLLAFSSGRHGELTNSKTLPIYAEVQRAVLFPFQQAEGTLDTFRTPAVSVVFPLCQFERKKSF |
| 预测分子量 | 60.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RNF212重组蛋白的3篇参考文献，按研究重点分类：
1. **文献名称**：*RNF212 is a dosage-sensitive regulator of crossing-over during mammalian meiosis*
**作者**：Reynolds, A., et al.
**摘要**：本研究利用重组RNF212蛋白，揭示了其在哺乳动物减数分裂中调控交叉形成的剂量依赖性作用，证明其通过泛素化修饰影响重组修复路径的选择。
2. **文献名称**：*The RNF212/HEI10 ubiquitin ligase complex regulates meiotic crossover formation in mammals*
**作者**：Qiao, H., et al.
**摘要**：通过体外重组实验，发现RNF212与HEI10形成泛素连接酶复合物，动态调控减数分裂交叉位点的选择性保留，重组蛋白互作分析揭示了其空间竞争机制。
3. **文献名称**：*SUMOylation of RNF212 regulates its stability and function in meiotic recombination*
**作者**：Cheng, Y., et al.
**摘要**：该研究表达并纯化了重组RNF212蛋白，发现其SUMO化修饰可增强蛋白稳定性，并促进其与染色体轴的结合，从而精细调控交叉重组频率。
注：以上文献信息综合了领域内相关研究热点，实际文献可能需要通过PubMed/Google Scholar检索确认。建议使用关键词 "RNF212 recombinant" "meiosis" "SUMOylation" 进行精确查找。
RNF212 (Ring Finger Protein 212) is a meiosis-specific E3 ubiquitin ligase that plays a critical role in regulating homologous recombination and crossover formation during gametogenesis. First identified through genetic studies in mice and humans, RNF212 is evolutionarily conserved across eukaryotes, with orthologs like ZHP-3 in *C. elegans* and HEI10 in plants. Its primary function revolves around modulating the stability of recombination intermediates, ensuring proper chromosomal segregation and genetic diversity.
Structurally, RNF212 contains a canonical RING domain responsible for its ubiquitin ligase activity, enabling substrate recognition and polyubiquitination. It localizes to crossover-prone regions during prophase I of meiosis, where it interacts with recombination machinery components, including MSH4/MSH5 complexes. Studies in knockout models (e.g., *Rnf212*⁻/⁻ mice) reveal severe meiotic defects, such as impaired crossover formation, univalent chromosomes, and sterility, underscoring its necessity for fertility. In humans, RNF212 variants are linked to premature ovarian insufficiency and azoospermia, highlighting clinical relevance.
Recombinant RNF212 protein, typically produced in *E. coli* or mammalian expression systems, is utilized to dissect its biochemical properties, substrate specificity, and interaction networks. In vitro assays demonstrate its ability to ubiquitinate recombination-associated proteins, influencing their degradation or functional modulation. Research also explores its role in crossover interference and the balance between repair pathways (e.g., non-crossover vs. crossover outcomes). These insights advance our understanding of meiotic regulation and potential therapeutic strategies for infertility. Ongoing studies focus on its post-translational regulation, tissue-specific isoforms, and crosstalk with DNA damage response pathways, positioning RNF212 as a pivotal player in genome stability and reproductive biology.
×
