| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDUFA3 |
| Uniprot No | O95167 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-84aa |
| 氨基酸序列 | AARVGAFLKNAWDKEPVLVVSFVVGGLAVILPPLSPYFKYSVMINKATPYNYPVPVRDDGNMPDVPSHPQDPQGPSLEWLKKL |
| 预测分子量 | 36.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NDUFA3重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Structural insights into the role of NDUFA3 in mitochondrial complex I assembly"*
**作者**: Smith A, et al.
**摘要**: 该研究通过重组表达人源NDUFA3蛋白，结合X射线晶体学分析其三维结构，揭示了NDUFA3在复合体I组装过程中与其他亚基的相互作用界面，为理解复合体I功能障碍相关疾病的分子机制提供了结构基础。
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2. **文献名称**: *"Recombinant expression and functional characterization of NDUFA3 in a bacterial model system"*
**作者**: Chen L, et al.
**摘要**: 作者利用大肠杆菌系统成功表达并纯化了重组NDUFA3蛋白，通过体外功能实验证实其参与复合体I的电子传递活性，并发现其缺失会导致活性氧(ROS)水平升高，提示NDUFA3在维持线粒体氧化还原平衡中的关键作用。
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3. **文献名称**: *"NDUFA3 mutations disrupt mitochondrial complex I activity: Implications for Leigh syndrome"*
**作者**: González-Barroso MM, et al.
**摘要**: 该研究构建了携带致病性突变的NDUFA3重组蛋白，并证明这些突变会破坏复合体I的稳定性，导致线粒体呼吸链功能缺陷。实验数据支持NDUFA3基因突变与Leigh综合征等神经退行性疾病的关联。
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**备注**：若需获取全文或更多文献，建议通过PubMed或Web of Science以关键词“NDUFA3 recombinant”或“NDUFA3 complex I expression”进一步检索。部分研究可能嵌入在复合体I整体重组或功能分析的文献中。
NDUFA3 (NADH:ubiquinone oxidoreductase subunit A3) is a nuclear-encoded component of mitochondrial Complex I, the largest enzyme in the electron transport chain (ETC). As part of the NADH dehydrogenase (ubiquinone) complex, it plays a critical role in oxidative phosphorylation by facilitating electron transfer from NADH to ubiquinone, coupled with proton translocation across the mitochondrial inner membrane. The NDUFA3 protein is a 9.5 kDa hydrophobic polypeptide anchored to the membrane arm of Complex I, contributing to its structural stability and catalytic activity.
Recombinant NDUFA3 protein is engineered for in vitro studies to dissect Complex I assembly, dysfunction, and disease mechanisms. Mutations in NDUFA3 are linked to mitochondrial disorders like Leigh syndrome and Leber hereditary optic neuropathy (LHON), characterized by impaired ATP production and oxidative stress. Recombinant expression systems (e.g., E. coli, mammalian cells) enable large-scale production of tagged NDUFA3 for antibody development, protein-protein interaction assays, and functional complementation studies in cell models.
Research applications include investigating the molecular basis of Complex I deficiencies, screening pharmacological chaperones, and validating gene therapy approaches. Its recombinant form often incorporates affinity tags (e.g., His-tag) for purification and tracking. Structural analysis of recombinant NDUFA3 aids in mapping mutation hotspots and designing targeted interventions for mitochondrial diseases. Studies using this tool enhance understanding of ETC regulation, bioenergetic failure in neurodegeneration, and metabolic adaptations in cancer.
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