| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MT4 |
| Uniprot No | P47944 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-62aa |
| 氨基酸序列 | MDPRECVCMSGGICMCGDNCKCTTCNCKTYWKSCCPCCPPGCAKCARGCICKGGSDKCSCCP |
| 预测分子量 | 22.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于MT4重组蛋白的参考文献示例(注：以下内容为虚构，仅作格式参考，实际文献请通过学术数据库查询)：
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1. **文献名称**：Expression and Purification of Recombinant MT4 Protein in Escherichia coli
**作者**：Chen L, Wang Y
**摘要**：本研究利用大肠杆菌表达系统成功表达并纯化了MT4重组蛋白，通过优化诱导条件和亲和层析技术获得高纯度蛋白，为后续功能研究奠定基础。
2. **文献名称**：Functional Characterization of MT4 Recombinant Protein in Tumor Angiogenesis
**作者**：Zhang R, et al.
**摘要**：探讨MT4重组蛋白在肿瘤血管生成中的作用，体外实验表明其通过调控VEGF信号通路抑制内皮细胞迁移，提示其潜在抗肿瘤应用价值。
3. **文献名称**：MT4 Recombinant Protein Enhances Wound Healing via Collagen Deposition
**作者**：Kim S, Patel D
**摘要**：在小鼠模型中验证MT4重组蛋白对创伤修复的影响，结果显示其通过促进胶原蛋白沉积加速伤口愈合，且无显著炎症反应。
4. **文献名称**：Structural Analysis of MT4 Recombinant Protein by Circular Dichroism
**作者**：Müller F, et al.
**摘要**：采用圆二色光谱技术解析MT4重组蛋白的二级结构，发现其具有典型的α-螺旋构象，并揭示pH值变化对其稳定性的影响。
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建议通过PubMed、Web of Science等平台，以关键词“MT4 recombinant protein”或“recombinant MT4 metallothionein”检索真实文献。
**Background of MT4 Recombinant Protein**
MT4. also known as metallothionein-4. is a member of the metallothionein (MT) family, a group of cysteine-rich, low-molecular-weight proteins renowned for their metal-binding capabilities. These proteins play critical roles in metal homeostasis, detoxification of heavy metals (e.g., cadmium, zinc, and copper), and protection against oxidative stress. MT4. specifically, is distinguished from other MT isoforms by its unique tissue expression patterns and structural features. It is predominantly expressed in stratified squamous epithelia, such as the skin, tongue, and esophagus, suggesting specialized functions in these tissues.
The recombinant MT4 protein is produced via genetic engineering techniques, where the MT4 gene is cloned and expressed in heterologous systems like *E. coli* or mammalian cell cultures. This allows large-scale production of the protein under controlled conditions. Recombinant MT4 retains the native protein's ability to bind metal ions through its conserved cysteine clusters, which form metal-thiolate complexes. These properties make it valuable for studying metal metabolism, cellular responses to metal toxicity, and redox regulation.
In research, MT4 recombinant protein is utilized to explore its potential therapeutic applications, such as mitigating heavy metal poisoning or addressing disorders linked to metal dysregulation. It also serves as a tool to investigate epithelial-specific defense mechanisms against environmental stressors. Additionally, its antioxidant properties are of interest in contexts like aging, inflammation, and cancer, where oxidative damage is a contributing factor.
The development of recombinant MT4 has advanced studies into its structure-function relationships, offering insights into how minor sequence variations among MT isoforms translate to diverse biological roles. Ongoing research aims to harness its metal-binding and cytoprotective traits for biomedical and industrial applications.
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