| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Mutc |
| Uniprot No | P16332 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-748aa |
| 氨基酸序列 | LHQQQPLHPEWAVLAKKQLKGKNPEDLIWHTPEGISIKPLYSRADTLDLPEELPGVKPFTRGPYPTMYTYRPWTIRQYAGFSTVEESNKFYKDNIKAGQQGLSVAFDLATHRGYDSDNPRVRGDVGMAGVAIDTVEDTKILFDGIPLEKMSVSMTMNGAVIPVLATFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFPPEPSMKIIADIFQYTAQHMPKFNSISISGYHMQEAGADAILELAYTIADGLEYCRTGLQAGLTIDEFAPRLSFFWGIGMNFYMEIAKMRAGRRLWAHLIEKMFQPKNSKSLLLRAHCQTSGWSLTEQDPYNNIVRTAIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSYMMESLTNDVYEAALKLIYEVEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDSVEVLAIDNTSVRKKQIEKLKKIKSSRDQALAEQCLSALTQCAASGDGNILALAVDAARARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVNKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGVSTLAAGHKTLVPELIKELTALGRPDILVMCGGVIPPQDYEFLYEVGVSNVFGPGTRIPRAAVQVLDDIEKCLAEKQQSV |
| 预测分子量 | 83.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MutC重组蛋白的3篇示例参考文献(注：以下为虚构示例，仅供参考)：
---
1. **文献名称**: *Expression and Functional Analysis of Recombinant MutC Protein in Mycobacterium tuberculosis*
**作者**: Smith, J. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了结核分枝杆菌MutC重组蛋白，证实其参与DNA错配修复机制，并通过体外实验验证了其增强基因组稳定性的功能。
2. **文献名称**: *MutC Recombinant Protein as a Novel Target for Cancer Immunotherapy*
**作者**: Li, X. & Wang, Y.
**摘要**: 通过体外实验证明，MutC重组蛋白可通过激活T细胞免疫应答抑制肿瘤生长，为开发基于MutC的癌症疫苗提供了理论基础。
3. **文献名称**: *Structural Characterization of MutC Recombinant Protein by X-ray Crystallography*
**作者**: Brown, K. et al.
**摘要**: 利用X射线晶体学解析了MutC重组蛋白的三维结构，揭示了其底物结合域的关键氨基酸残基，为设计靶向抑制剂提供了结构依据。
---
如需真实文献，建议使用关键词 **"MutC recombinant protein"** 或 **"MutC protein DNA repair"** 在PubMed或Google Scholar中检索，并核实具体研究领域(如微生物学、癌症或酶工程)。
**Background of MutC Recombinant Protein**
MutC recombinant protein is a genetically engineered protein derived from the MutC gene, which encodes a critical component in bacterial DNA mismatch repair (MMR) systems. Initially identified in *Escherichia coli*, MutC (or MutS homologs in other species) plays a pivotal role in recognizing and initiating correction of base-pair mismatches or small insertion/deletion loops during DNA replication, ensuring genomic stability. The recombinant form is produced via molecular cloning, where the MutC gene is inserted into expression vectors (e.g., plasmids) and expressed in host systems like *E. coli* or yeast. This allows large-scale production of purified MutC protein for research and applications.
Recombinant MutC retains the functional domains of the native protein, including ATPase activity and DNA-binding specificity, making it valuable for studying MMR mechanisms, mutagenesis, and genomic integrity in vitro. Its applications extend to biotechnology, such as enhancing DNA replication fidelity in engineered organisms, or diagnostic tools for detecting mutations. Additionally, MutC variants with modified binding affinities or thermostability are explored for industrial uses, including DNA repair enzyme kits or biosensors.
Recent studies also investigate MutC's potential in cancer research, as MMR defects correlate with tumorigenesis. Recombinant MutC aids in screening for MMR-deficient cancers or evaluating chemotherapeutic agents targeting DNA repair pathways. Overall, MutC recombinant protein bridges fundamental research and practical innovations, underscoring its significance in molecular biology and genetic engineering.
×
