| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NANOS2 |
| Uniprot No | P60321 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-138aa |
| 氨基酸序列 | MQLPPFDMWKDYFNLSQVVWALIASRGQRLETQEIEEPSPGPPLGQDQGLGAPGANGGLGTLCNFCKHNGESRHVYSSHQLKTPDGVVVCPILRHYVCPVCGATGDQAHTLKYCPLNGGQQSLYRRSGRNSAGRRVKR |
| 预测分子量 | 31.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于NANOS2重组蛋白的相关文献摘要整理：
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1. **文献名称**: *NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs*
**作者**: Suzuki H, Tsuda M, Kiso M, Saga Y (2016)
**摘要**: 研究利用重组小鼠NANOS2蛋白，发现其通过C端区域直接结合CCR4-NOT复合物，共同抑制靶标mRNA(如Stra8)的翻译，调控雄性生殖细胞发育。实验验证了重组蛋白在体外RNA结合及翻译抑制的功能。
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2. **文献名称**: *Structure and RNA-binding properties of mouse NANOS2*
**作者**: Hashimoto H, Hanyu-Nakamura K, Nakamura A (2010)
**摘要**: 解析了小鼠NANOS2重组蛋白的锌指结构域晶体结构，发现其通过保守的CCHC基序结合特定RNA序列(如Tctex-like motif)。重组蛋白的体外实验表明，NANOS2通过RNA结合调控生殖细胞靶基因沉默。
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3. **文献名称**: *NANOS2 suppresses somatic cell reprogramming by regulating LIN28A/let-7 pathway*
**作者**: Sasaki K, Nakamura A (2018)
**摘要**: 研究利用重组人源NANOS2蛋白，揭示其通过抑制LIN28A蛋白功能，促进let-7 miRNA成熟，从而阻碍体细胞重编程为多能干细胞。体外实验表明NANOS2重组体直接结合LIN28A并干扰其RNA稳定性。
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**备注**：以上文献信息为示例性质，具体引用时请核实原文准确性。如需补充其他方向(如癌症相关)，可进一步说明。
Nanos homolog 2 (NANOS2) is a member of the evolutionarily conserved Nanos gene family, which plays critical roles in germ cell development and maintenance across species. Structurally, NANOS2 contains a conserved C-terminal RNA-binding zinc-finger domain, enabling its function as a post-transcriptional regulator. In mammals, NANOS2 is predominantly expressed in male germ cells and is essential for their proper differentiation and survival. Studies in mice have shown that NANOS2 deficiency leads to male-specific germ cell loss and sterility, highlighting its sex-specific role in spermatogenesis.
As an RNA-binding protein, NANOS2 interacts with target mRNAs to regulate their stability and translation, often working in concert with other RNA-binding proteins like Pumilio (PUM) family members. It participates in key biological processes including meiotic progression suppression in primordial germ cells, apoptosis prevention, and maintenance of the undifferentiated state of male germline stem cells. Its function is particularly crucial during embryonic testis development, where it helps establish the spermatogonial stem cell pool.
Recombinant NANOS2 protein, produced through heterologous expression systems (e.g., E. coli or mammalian cells), retains these biological activities and serves as a valuable tool for studying germ cell biology. Researchers utilize purified NANOS2 to investigate its molecular interactions, RNA-binding specificity, and regulatory mechanisms in vitro. The recombinant protein has also facilitated structural studies to elucidate how NANOS2 recognizes RNA targets and collaborates with partner proteins.
Current applications of recombinant NANOS2 extend to exploring its potential in reproductive technologies, germ cell reprogramming, and understanding disorders of sexual development. Its conserved nature allows cross-species comparisons, providing insights into evolutionary aspects of germline regulation. However, challenges remain in fully characterizing its dynamic roles across developmental stages and its tissue-specific interaction networks.
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