| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANOS1 |
| Uniprot No | P23352 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-680aa |
| 氨基酸序列 | AGPGAA AARRLDESLS AGSVQRARCA SRCLSLQITR ISAFFQHFQN NGSLVWCQNH KQCSKCLEPC KESGDLRKHQ CQSFCEPLFP KKSYECLTSC EFLKYILLVK QGDCPAPEKA SGFAAACVES CEVDNECSGV KKCCSNGCGH TCQVPKTLYK GVPLKPRKEL RFTELQSGQL EVKWSSKFNI SIEPVIYVVQ RRWNYGIHPS EDDATHWQTV AQTTDERVQL TDIRPSRWYQ FRVAAVNVHG TRGFTAPSKH FRSSKDPSAP PAPANLRLAN STVNSDGSVT VTIVWDLPEE PDIPVHHYKV FWSWMVSSKS LVPTKKKRRK TTDGFQNSVI LEKLQPDCDY VVELQAITYW GQTRLKSAKV SLHFTSTHAT NNKEQLVKTR KGGIQTQLPF QRRRPTRPLE VGAPFYQDGQ LQVKVYWKKT EDPTVNRYHV RWFPEACAHN RTTGSEASSG MTHENYIILQ DLSFSCKYKV TVQPIRPKSH SKAEAVFFTT PPCSALKGKS HKPVGCLGEA GHVLSKVLAK PENLSASFIV QDVNITGHFS WKMAKANLYQ PMTGFQVTWA EVTTESRQNS LPNSIISQSQ ILPSDHYVLT VPNLRPSTLY RLEVQVLTPG GEGPATIKTF RTPELPPSSA HRSHLKHRHP HHYKPSPERY |
| 预测分子量 | 76,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ANOS1重组蛋白的参考文献概览：
1. **文献名称**: *ANOS1 mutations induce ectopic activity of a new neuronal CXCL12 secretory system in the developing human forebrain*
**作者**: Cariboni A, et al.
**摘要**: 该研究通过重组ANOS1蛋白实验，揭示其突变导致CXCL12信号通路异常，影响胚胎期神经元迁移，进而阐明Kallmann综合征的致病机制。
2. **文献名称**: *Structural basis of anosmin-1 recognition by the neuronal receptor FGFR1*
**作者**: Hu Y, et al.
**摘要**: 利用重组ANOS1蛋白进行晶体结构解析，发现其通过特异性结合FGFR1受体调控神经元轴突导向，为相关生殖障碍疾病提供分子机制解释。
3. **文献名称**: *Functional characterization of missense mutations in ANOS1 gene via recombinant protein expression*
**作者**: Soussi-Yanicostas N, et al.
**摘要**: 通过大肠杆菌表达系统制备重组ANOS1突变体蛋白，证实其与细胞外基质互作能力受损，导致嗅觉神经元发育异常。
注：上述文献为虚拟示例，实际文献需通过PubMed或Web of Science检索关键词“ANOS1 recombinant protein”获取。建议结合具体研究方向筛选近年高被引论文。
ANOS1 recombinant protein is derived from the ANOS1 gene (previously known as KAL1), located on the X chromosome (Xp22.31). This gene encodes anosmin-1. a secreted extracellular matrix-associated glycoprotein critical for neuronal development and olfactory system morphogenesis. Structurally, anosmin-1 contains a cysteine-rich N-terminal region, a whey acidic protein (WAP) domain, and four fibronectin type III (FN3) repeats, enabling interactions with cell surface receptors and extracellular components.
Mutations in ANOS1 are linked to X-linked Kallmann syndrome, characterized by congenital hypogonadotropic hypogonadism and anosmia (loss of smell). The protein facilitates embryonic migration of gonadotropin-releasing hormone (GnRH) neurons and olfactory axon guidance through interactions with fibroblast growth factor receptor 1 (FGFR1) and heparan sulfate proteoglycans. Its role in neural circuit formation extends to broader developmental processes, including kidney morphogenesis.
Recombinant ANOS1 protein is typically produced using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications. This engineered protein retains functional domains necessary for studying cellular signaling pathways, neuronal migration assays, and disease modeling. Researchers utilize it to investigate molecular mechanisms underlying Kallmann syndrome, test therapeutic strategies, and explore its potential in regenerative medicine. Its applications extend to drug screening platforms and structural studies analyzing ligand-receptor interactions. As a research tool, recombinant ANOS1 provides insights into neurodevelopmental disorders and sex-specific disease manifestations while maintaining relevance for both basic science and translational investigations.
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