| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITPKB |
| Uniprot No | P27987 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 442-946aa |
| 氨基酸序列 | RVEGGSPTLGLLGGSPSAQPGTGNVEAGIPSGRMLEPLPCWDAAKDLKEPQCPPGDRVGVQPGNSRVWQGTMEKAGLAWTRGTGVQSEGTWESQRQDSDALPSPELLPQDPDKPFLRKACSPSNIPAVIITDMGTQEDGALEETQGSPRGNLPLRKLSSSSASSTGFSSSYEDSEEDISSDPERTLDPNSAFLHTLDQQKPRVSKSWRKIKNMVHWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMVDVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGIRTYLEEELTKARKKPSLRKDMYQKMIEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGTVNRDFKKTKTREQVTEAFREFTKGNHNILIAYRDRLKAIRTTLEVSPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTLQHDVPWQEGNREDGYLSGLNNLVDILTEMSQDAPLA |
| 预测分子量 | 72.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITPKB重组蛋白的3篇参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*"Expression and Functional Analysis of Recombinant Human ITPKB in T-Cell Signaling"*
**作者**：Johnson, R. et al.
**摘要**：研究通过大肠杆菌系统成功表达重组人源ITPKB蛋白，并验证其激酶活性，证明其在T细胞受体信号传导中通过调控IP3代谢影响钙离子通道功能。
2. **文献名称**：*"Structural Insights into ITPKB Catalytic Mechanism Using Recombinant Protein Crystallography"*
**作者**：Li, X. et al.
**摘要**：利用昆虫细胞表达系统获得高纯度重组ITPKB蛋白，通过X射线晶体学解析其三维结构，揭示了其底物结合域的关键氨基酸残基及催化机制。
3. **文献名称**：*"ITPKB Deficiency and Neurodegeneration: Role of Recombinant ITPKB in Neuronal Survival"*
**作者**：Wang, Y. et al.
**摘要**：研究构建重组ITPKB蛋白并用于体外神经元模型，证实其通过调节IP4水平抑制内质网应激，从而延缓神经退行性疾病进程。
如需具体文献，建议通过PubMed或Google Scholar以“ITPKB recombinant protein”或“ITPKB expression”等关键词检索。
ITPKB (Inositol-trisphosphate 3-kinase B) is a lipid kinase that plays a critical role in cellular signaling by phosphorylating inositol 1.4.5-trisphosphate (IP3) to generate inositol 1.3.4.5-tetrakisphosphate (IP4). This enzymatic activity regulates intracellular calcium signaling, which is essential for diverse physiological processes, including immune response modulation, neuronal function, and metabolic regulation. ITPKB is particularly important in immune cells, where it fine-tunes signaling pathways involved in T-cell development, activation, and apoptosis. Dysregulation of ITPKB has been linked to autoimmune diseases, neurodegenerative disorders, and cancer, highlighting its therapeutic potential.
Recombinant ITPKB protein refers to the engineered form of the enzyme produced using heterologous expression systems, such as bacterial, insect, or mammalian cells. This approach allows for large-scale production of highly pure, functional ITPKB, enabling detailed biochemical and structural studies. Researchers utilize recombinant ITPKB to investigate its enzymatic kinetics, substrate specificity, and interactions with regulatory proteins or small molecules. In drug discovery, it serves as a tool for screening inhibitors or activators targeting ITPKB activity, with applications in developing therapies for immune-related diseases or cancers. Additionally, recombinant ITPKB is used in cellular assays to study calcium signaling dynamics and validate genetic findings in disease models. Its availability accelerates both basic research and translational efforts aimed at understanding ITPKB’s role in health and disease.
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