| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IP6K1 |
| Uniprot No | Q92551 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-441aa |
| 氨基酸序列 | MCVCQTMEVGQYGKNASRAGDRGVLLEPFIHQVGGHSSMMRYDDHTVCKP LISREQRFYESLPPEMKEFTPEYKGVVSVCFEGDSDGYINLVAYPYVESE TVEQDDTTEREQPRRKHSRRSLHRSGSGSDHKEEKASLSLETSESSQEAK SPKVELHSHSEVPFQMLDGNSGLSSEKISHNPWSLRCHKQQLSRMRSESK DRKLYKFLLLENVVHHFKYPCVLDLKMGTRQHGDDASAEKAARQMRKCEQ STSATLGVRVCGMQVYQLDTGHYLCRNKYYGRGLSIEGFRNALYQYLHNG LDLRRDLFEPILSKLRGLKAVLERQASYRFYSSSLLVIYDGKECRAESCL DRRSEMRLKHLDMVLPEVASSCGPSTSPSNTSPEAGPSSQPKVDVRMIDF AHSTFKGFRDDPTVHDGPDRGYVFGLENLISIMEQMRDENQ |
| 预测分子量 | 66 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IP6K1重组蛋白的3篇代表性文献的概括(注：文献为示例性内容，具体引用需核实真实来源)：
1. **"Recombinant human IP6K1: expression, purification, and kinetic characterization"**
- **作者**: Smith A, et al.
- **摘要**: 本研究报道了在大肠杆菌系统中高效表达并纯化重组人源IP6K1蛋白的方法，通过亲和层析和凝胶过滤获得高纯度蛋白。进一步分析了其酶动力学特性，证实IP6K1对肌醇六磷酸(IP6)的磷酸化活性及对ATP的依赖性。
2. **"Structural insights into IP6K1 catalysis through recombinant protein crystallization"**
- **作者**: Chen L, et al.
- **摘要**: 利用重组IP6K1蛋白进行X射线晶体学研究，解析了其与底物IP6/ATP结合的复合物结构，揭示了关键催化位点(如磷酸基团转移的活性中心)，为靶向IP6K1的药物设计提供了结构基础。
3. **"IP6K1 regulates mitochondrial dynamics via recombinant protein interaction assays"**
- **作者**: Gupta R, et al.
- **摘要**: 通过体外重组IP6K1蛋白与线粒体相关蛋白(如VDAC1)的互作实验，发现IP6K1通过生成5-IP7调控线粒体膜稳定性，并影响细胞凋亡信号通路，提示其在代谢疾病中的潜在作用。
4. **"Development of a high-throughput assay for IP6K1 activity using recombinant protein"**
- **作者**: Wang Y, et al.
- **摘要**: 基于重组IP6K1蛋白建立了荧光标记的体外高通量酶活检测体系，用于筛选小分子抑制剂，并验证了多个候选化合物对IP6K1的抑制效果，为癌症治疗研究提供了工具。
建议通过PubMed或Web of Science以“IP6K1 recombinant protein”为关键词检索最新文献获取具体信息。
IP6K1 (Inositol Hexakisphosphate Kinase 1) is a member of the inositol phosphate kinase family that catalyzes the phosphorylation of inositol hexakisphosphate (IP6) to generate 5-diphosphoinositol 1.2.3.4.6-pentakisphosphate (5-IP7 or IP7). This enzyme plays a critical role in regulating cellular processes such as apoptosis, autophagy, vesicular trafficking, and metabolic signaling. IP6K1 is ubiquitously expressed, with high levels observed in the brain, liver, and pancreatic β-cells, where it modulates insulin secretion and glucose homeostasis. Dysregulation of IP6K1 has been implicated in metabolic disorders (e.g., obesity, diabetes), neurodegenerative diseases, and cancer progression.
Recombinant IP6K1 proteins are engineered to study its enzymatic activity, structure-function relationships, and interactions with downstream targets (e.g., p53. AMPK). These proteins are typically produced in *E. coli* or mammalian expression systems, often fused with tags (e.g., His-tag, GST) for purification and detection. Structural studies using recombinant IP6K1 have revealed insights into its ATP-binding pocket and substrate specificity, aiding in the design of selective inhibitors (e.g., TNP). Such inhibitors are explored as therapeutic agents for metabolic diseases and cancer.
Research on recombinant IP6K1 also highlights its role in modulating cellular energy dynamics by competing with ATP-dependent enzymes, linking inositol pyrophosphates to metabolic stress responses. Its dual role as a kinase and a scaffolding protein underscores its versatility in signaling networks. Current studies focus on tissue-specific IP6K1 isoforms, post-translational modifications, and its crosstalk with other signaling pathways, positioning it as a potential biomarker or drug target in precision medicine.
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