| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ISX |
| Uniprot No | Q2M1V0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-245aa |
| 氨基酸序列 | MCAEVGPALCRGMERNSLGCCEAPKKLSLSFSIEAILKRPARRSDMDRPEGPGEEGPGEAAASGSGLEKPPKDQPQEGRKSKRRVRTTFTTEQLHELEKIFHFTHYPDVHIRSQLAARINLPEARVQIWFQNQRAKWRKQEKIGNLGAPQQLSEASVALPTNLDVAGPTWTSTALRRLAPPTSCCPSAQDQLASAWFPAWITLLPAHPWETQPVPGLPIHQTCIPVLCILPPPHPKWGSICATST |
| 预测分子量 | 43.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ISX重组蛋白研究的模拟参考文献示例(仅供参考)：
1. **《Cloning and Expression of Recombinant ISX Protein in E. coli》**
- 作者：Zhang L, et al.
- 摘要：研究成功克隆ISX基因并在大肠杆菌中实现重组表达，优化了诱导条件以提高可溶性蛋白产量，并通过Western blot验证了蛋白特异性。
2. **《Functional Characterization of ISX Recombinant Protein in Tumor Microenvironment Regulation》**
- 作者：Wang Y, et al.
- 摘要：分析了ISX重组蛋白的抗肿瘤活性，发现其通过抑制血管生成因子VEGF和调节T细胞活性抑制小鼠模型中的肿瘤生长。
3. **《Structural Analysis of ISX Recombinant Protein by X-ray Crystallography》**
- 作者：Kim S, et al.
- 摘要：首次解析了ISX重组蛋白的晶体结构，揭示了其与配体结合的活性位点，为靶向药物设计提供了结构基础。
4. **《ISX Recombinant Protein Enhances Antigen Presentation in Dendritic Cells》**
- 作者：Müller R, et al.
- 摘要：证明ISX重组蛋白可增强树突状细胞的抗原呈递能力，并通过TLR4/NF-κB通路激活免疫应答，提示其作为疫苗佐剂的潜力。
**说明**：以上文献为模拟示例，实际研究需通过PubMed、Google Scholar等平台检索最新文献。建议使用关键词“ISX recombinant protein”、“ISX gene function”或结合具体研究领域(如癌症、免疫)进行精准检索。
**Background of ISX Recombinant Protein**
ISX (Intestinal Specific Homeobox) is a transcription factor encoded by the *ISX* gene, primarily involved in regulating intestinal development, differentiation, and homeostasis. It plays a critical role in maintaining the balance between intestinal stem cell proliferation and differentiation, influencing nutrient absorption and metabolic processes. Dysregulation of ISX has been linked to gastrointestinal disorders, metabolic syndromes, and colorectal cancer, highlighting its physiological and pathological significance.
Recombinant ISX protein is produced using genetic engineering techniques, where the *ISX* gene is cloned into expression vectors and expressed in host systems like *E. coli*, yeast, or mammalian cells. This allows large-scale production of purified, functional ISX protein for research and therapeutic applications. The recombinant form retains the DNA-binding ability of the native protein, enabling studies on its interaction with target genes, such as those involved in carotenoid metabolism (*e.g., BCO1*) or intestinal cell fate regulation.
In research, ISX recombinant protein is utilized to investigate molecular mechanisms underlying intestinal diseases, cancer progression, and metabolic dysfunctions. It serves as a tool for *in vitro* assays, *in vivo* models, and drug discovery targeting ISX-associated pathways. Additionally, it aids in developing diagnostic biomarkers or therapies aimed at modulating ISX activity. Challenges in production include ensuring proper post-translational modifications (achieved via mammalian expression systems) and maintaining structural stability.
Overall, ISX recombinant protein is a vital resource for advancing understanding of gut biology and related pathologies, bridging basic science with translational applications.
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