| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R2C |
| Uniprot No | Q9Y2T4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-447aa |
| 氨基酸序列 | MGEDTDTRKINHSFLRDHSYVTEADIISTVEFNHTGELLATGDKGGRVVIFQREPESKNAPHSQGEYDVYSTFQSHEPEFDYLKSLEIEEKINKIKWLPQQNAAHSLLSTNDKTIKLWKITERDKRPEGYNLKDEEGKLKDLSTVTSLQVPVLKPMDLMVEVSPRRIFANGHTYHINSISVNSDCETYMSADDLRINLWHLAITDRSFNIVDIKPANMEDLTEVITASEFHPHHCNLFVYSSSKGSLRLCDMRAAALCDKHSKLFEEPEDPSNRSFFSEIISSVSDVKFSHSGRYMLTRDYLTVKVWDLNMEARPIETYQVHDYLRSKLCSLYENDCIFDKFECAWNGSDSVIMTGAYNNFFRMFDRNTKRDVTLEASRESSKPRAVLKPRRVCVGGKRRRDDISVDSLDFTKKILHTAWHPAENIIAIAATNNLYIFQDKVNSDMH |
| 预测分子量 | 58.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP2R2C重组蛋白的3篇参考文献示例(注：文献为虚构示例，实际研究中请以真实数据库检索结果为准)：
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1. **标题**: *Structural and Functional Characterization of Recombinant PPP2R2C in the PP2A Holoenzyme*
**作者**: Li, X. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组PPP2R2C蛋白，通过冷冻电镜解析了其与PP2A核心酶的复合物结构，揭示了其特异性结合模式及对底物选择性的调控机制。
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2. **标题**: *PPP2R2C Recombinant Protein as a Novel Biomarker in Breast Cancer*
**作者**: Garcia, S.M. & Zhang, Y.
**摘要**: 文章报道了通过哺乳动物细胞系重组表达PPP2R2C，发现其在乳腺癌组织中表达显著下调，并通过体外实验证明其过表达可抑制肿瘤细胞增殖，提示其潜在抑癌功能。
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3. **标题**: *High-Yield Expression of Human PPP2R2C in Insect Cells for Kinase Assay Applications*
**作者**: Kumar, R. et al.
**摘要**: 利用杆状病毒-昆虫细胞系统高效表达PPP2R2C重组蛋白，优化纯化工艺后获得高纯度蛋白，并验证其在体外激酶活性检测中对PP2A酶活性的调节作用。
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**提示**：实际文献检索建议使用PubMed或Google Scholar，结合关键词“PPP2R2C recombinant”“PP2A regulatory subunit”等，并筛选近年研究以获取最新进展。
**Background of PPP2R2C Recombinant Protein**
PPP2R2C (Protein Phosphatase 2 Regulatory Subunit Bgamma) is a key component of the Protein Phosphatase 2A (PP2A) holoenzyme, a highly conserved serine/threonine phosphatase involved in regulating diverse cellular processes. PP2A functions as a heterotrimer comprising a structural (A), catalytic (C), and regulatory (B) subunit. The PPP2R2C gene encodes the Bgamma regulatory subunit, part of the B55 subfamily, which determines substrate specificity, subcellular localization, and enzymatic activity of the PP2A complex.
Structurally, PPP2R2C contains multiple WD40 repeats, facilitating protein-protein interactions critical for recruiting PP2A to specific targets. It plays a role in cell cycle progression, apoptosis, DNA damage response, and signal transduction pathways (e.g., Wnt, Akt/mTOR). Dysregulation of PP2A, including its B subunits, is implicated in cancers, neurodegenerative diseases, and developmental disorders.
Recombinant PPP2R2C protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional subunits for mechanistic studies. This tool enables researchers to dissect PP2A assembly, substrate recognition, and regulatory mechanisms in vitro or in cellular models. Its applications span drug discovery, structural biology (e.g., crystallography to map binding interfaces), and disease modeling, particularly in contexts where PP2A activity is perturbed. Recent studies highlight PPP2R2C's potential as a biomarker or therapeutic target, given its altered expression in malignancies like glioblastoma and leukemia. However, its precise roles in tissue-specific signaling and disease pathogenesis remain under investigation, necessitating further exploration using recombinant protein-based approaches.
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