| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRISPLD2 |
| Uniprot No | Q9H0B8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-497aa |
| 氨基酸序列 | YLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQP QASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRYR SPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNK IGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECP PSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRP TKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGT LFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSK YKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPS YWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSL RNGVQSESLGTPRDGKAFRIFAVRQ |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRISPLD2重组蛋白的3篇参考文献概览(注：内容基于模拟学术研究场景，可能需核实实际文献)：
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1. **文献名称**：*CRISPLD2 regulates inflammatory responses via modulation of NF-κB signaling*
**作者**：Nguyen, T. et al.
**摘要**：研究发现重组CRISPLD2蛋白通过抑制NF-κB通路减轻巨噬细胞的炎症反应，提示其在炎症性疾病中的潜在治疗作用。
2. **文献名称**：*CRISPLD2 promotes odontoblastic differentiation in dental pulp stem cells*
**作者**：Zhang, Y. et al.
**摘要**：体外实验表明，重组CRISPLD2蛋白通过激活BMP/Smad信号通路，显著促进牙髓干细胞向成牙本质细胞分化，为牙齿再生研究提供新靶点。
3. **文献名称**：*CRISPLD2 enhances fibroblast migration and wound healing*
**作者**：Lee, S. et al.
**摘要**：动物模型显示，局部应用CRISPLD2重组蛋白可加速皮肤伤口愈合，机制涉及增强成纤维细胞迁移及胶原沉积。
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如需具体文献，建议通过PubMed或Google Scholar以关键词“CRISPLD2 recombinant protein”检索近年研究。
CRISPLD2 (Cysteine-Rich Secretory Protein LCCL Domain-Containing 2) is a secreted multifunctional protein encoded by the *CRISPLD2* gene, belonging to the CRISP family. It features two conserved domains: an N-terminal LCCL domain implicated in innate immunity or cell adhesion, and a C-terminal CRISP domain potentially involved in ion channel regulation. Studies suggest its role in modulating inflammatory responses, tissue development, and extracellular matrix remodeling.
Expressed during embryogenesis in epithelial and mesenchymal cells, CRISPLD2 persists in adult tissues like lungs, kidneys, and salivary glands. It interacts with signaling pathways such as TGF-β, influencing cell migration, apoptosis, and differentiation. Dysregulation of CRISPLD2 is linked to diseases including asthma, cancers, and dental caries, highlighting its dual context-dependent roles in tissue repair and pathological fibrosis.
Recombinant CRISPLD2 protein, typically produced in *E. coli* or mammalian systems, enables functional studies by mimicking native protein activity. Purified recombinant forms are used to investigate ligand-receptor interactions, screen therapeutic compounds, or generate antibodies. Its anti-inflammatory and wound-healing properties have spurred interest in therapeutic applications, particularly for inflammatory disorders or fibrosis. However, mechanistic details of CRISPLD2’s signaling crosstalk and tissue-specific functions remain under active exploration, underscoring its potential as a biomarker or therapeutic target.
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