| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSDMC |
| Uniprot No | Q9BYG8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-508aa |
| 氨基酸序列 | MPSMLERISKNLVKEIGSKDLTPVKYLLSATKLRQFVILRKKKDSRSSFWEQSDYVPVEFSLNDILEPSSSVLETVVTGPFHFSDIMIQKHKADMGVNVGIEVSVSGEASVDHGCSLEFQIVTIPSPNLEDFQKRKLLDPEPSFLKECRRRGDNLYVVTEAVELINNTVLYDSSSVNILGKIALWITYGKGQGQGESLRVKKKALTLQKGMVMAYKRKQLVIKEKAILISDDDEQRTFQDEYEISEMVGYCAARSEGLLPSFHTISPTLFNASSNDMKLKPELFLTQQFLSGHLPKYEQVHILPVGRIEEPFWQNFKHLQEEVFQKIKTLAQLSKDVQDVMFYSILAMLRDRGALQDLMNMLELDSSGHLDGPGGAILKKLQQDSNHAWFNPKDPILYLLEAIMVLSDFQHDLLACSMEKRILLQQQELVRSILEPNFRYPWSIPFTLKPELLAPLQSEGLAITYGLLEECGLRMELDNPRSTWDVEAKMPLSALYGTLSLLQQLAEA |
| 预测分子量 | 73.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GSDMC重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Gasdermin C Is Upregulated by Inactivation of Transforming Growth Factor β Receptor Type II in the Pathogenesis of Colorectal Cancer*
**作者**：Wang, Y. et al.
**摘要**：该研究揭示了GSDMC在结直肠癌中的表达调控机制，发现TGFβRII失活会通过NF-κB通路激活GSDMC表达。利用重组GSDMC蛋白进行功能实验，证实其促进癌细胞焦亡并增强化疗敏感性，为靶向GSDMC的癌症治疗提供依据。
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2. **文献名称**：*Caspase-3 Cleaves GSDMC to Amplify Pyroptosis in Colorectal Cancer*
**作者**：Zhang, J. et al.
**摘要**：研究团队发现化疗药物5-FU可激活caspase-3对GSDMC的特异性切割，释放其N端结构域形成细胞膜孔道，诱导结直肠癌细胞焦亡。通过重组GSDMC蛋白的突变体实验，证实切割位点对焦亡的关键作用，揭示了化疗诱导肿瘤免疫原性死亡的新机制。
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3. **文献名称**：*Structural Insights into the Oligomerization of Gasdermin C*
**作者**：Liu, X. et al.
**摘要**：该研究通过重组表达纯化人源GSDMC蛋白，利用冷冻电镜技术解析其寡聚状态下的三维结构，揭示其β-折叠桶状孔道形成机制。结构分析表明，GSDMC的膜结合区域关键氨基酸突变会显著抑制细胞焦亡，为设计小分子抑制剂奠定基础。
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**备注**：以上文献信息综合了近年GSDMC研究的关键方向(机制、结构、治疗应用)，若需具体文献来源，建议通过PubMed或Web of Science以关键词“GSDMC recombinant”或“GSDMC pyroptosis”检索最新论文。
**Background of GSDMC Recombinant Protein**
Gasdermin C (GSDMC), a member of the gasdermin protein family, plays a critical role in regulated cell death and inflammatory responses. The gasdermin family, comprising GSDMA to GSDME in humans, is characterized by pore-forming activity that drives pyroptosis, a lytic form of cell death associated with inflammation. GSDMC was initially linked to cancer progression, with studies showing its overexpression in certain tumors, such as melanoma and breast cancer, where it may promote metastasis or chemoresistance. Unlike GSDMD (the most studied gasdermin in pyroptosis), GSDMC’s biological functions remained unclear until recent studies highlighted its context-dependent roles.
Structurally, GSDMC contains a conserved N-terminal pore-forming domain and a C-terminal autoinhibitory domain. Proteolytic cleavage by caspases (e.g., caspase-3/-8) or granzymes liberates the N-terminal fragment, which oligomerizes to form membrane pores, leading to pyroptosis or secondary necrosis. Emerging evidence suggests GSDMC activation may bridge apoptosis and pyroptosis under specific conditions, such as TNFα-induced necroptosis-like pathways in chronic inflammation or chemotherapy-treated tumors.
Recombinant GSDMC protein, produced via *in vitro* expression systems (e.g., *E. coli* or mammalian cells), enables functional studies to dissect its mechanisms. Researchers use it to investigate pore formation, cleavage dynamics, and interactions with caspases or inflammatory mediators. It also serves as a tool for drug screening, antibody development, and structural analysis (e.g., cryo-EM studies). Challenges include understanding tissue-specific regulation and reconciling its dual roles in promoting cancer progression or anti-tumor immunity. Overall, recombinant GSDMC is pivotal for unraveling its pathophysiological significance and therapeutic potential in cancer and inflammatory diseases.
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