| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | P2RX6 |
| Uniprot No | O15547 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-441aa |
| 氨基酸序列 | MCPQLAGAGSMGSPGATTGWGLLDYKTEKYVMTRNWRVGALQRLLQFGIVVYVVGWALLAKKGYQERDLEPQFSIITKLKGVSVTQIKELGNRLWDVADFVKPPQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANCWVDEDCPEGEGGTHSHGVKTGQCVVFNGTHRTCEIWSWCPVESGVVPSRPLLAQAQNFTLFIKNTVTFSKFNFSKSNALETWDPTYFKHCRYEPQFSPYCPVFRIGDLVAKAGGTFEDLALLGGSVGIRVHWDCDLDTGDSGCWPHYSFQLQEKSYNFRTATHWWEQPGVEARTLLKLYGIRFDILVTGQAGKFGLIPTAVTLGTGAAWLGVVTFFCDLLLLYVDREAHFYWRTKYEEAKAPKATANSVWRELALASQARLAECLRRSSAPAPTATAAGSQTQTPGWPCPSSDTHLPTHSGSL |
| 预测分子量 | 48,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于P2RX6重组蛋白的3篇参考文献及其摘要概括：
1. **"Functional characterization of recombinant P2X6 receptors in ATP-gated cation channel activity"**
- **Authors**: Smith A, Jones B, et al.
- **摘要**: 本研究利用哺乳动物表达系统成功表达并纯化了重组P2X6受体蛋白，证实其在ATP激活下具有低亲和力的阳离子通道功能，并发现其与其他P2X亚基(如P2X4)形成异源三聚体时可增强通道活性。
2. **"Expression and purification of the human P2X6 receptor in insect cells for structural analysis"**
- **Authors**: Chen L, Wang H, et al.
- **摘要**: 通过杆状病毒-昆虫细胞系统高效表达人源P2X6重组蛋白，优化纯化流程后获得高纯度蛋白，初步结晶实验为解析其三维结构及配体结合机制奠定了基础。
3. **"Role of P2X6 receptor in airway inflammation: Insights from recombinant protein knockdown models"**
- **Authors**: Tanaka K, Suzuki M, et al.
- **摘要**: 利用重组P2X6蛋白构建的基因沉默模型发现，P2X6在哮喘小鼠气道炎症中通过调控ATP信号通路促进炎性细胞因子的释放，提示其作为潜在治疗靶点。
*注：以上文献信息为模拟生成，实际研究中建议通过PubMed或Web of Science检索最新论文。若需真实文献，可提供具体研究方向进一步筛选。*
P2RX6. a member of the P2X purinergic receptor family, is an ATP-gated ion channel involved in cellular signaling pathways triggered by extracellular adenosine triphosphate (ATP). As part of the P2X receptor subfamily, P2RX6 is characterized by its trimeric structure and role in mediating cation flux (Na⁺, K⁺, Ca²⁺) across cell membranes. Unlike other P2X receptors (e.g., P2RX7), P2RX6 exhibits unique functional properties, including limited ATP sensitivity and a propensity to form heteromeric complexes with other P2X subtypes (e.g., P2RX4 or P2RX7), which may modulate its activity and physiological roles.
Recombinant P2RX6 protein is engineered for in vitro studies to overcome challenges in isolating native receptors, such as low abundance and tissue-specific expression variability. Produced via heterologous expression systems (e.g., HEK293 or CHO cells), the recombinant form allows precise investigation of its biophysical properties, ligand interactions, and signaling mechanisms. Structural studies using recombinant P2RX6 have helped map ATP-binding domains and ion-conducting pathways, though full structural resolution remains limited compared to other P2X receptors.
Functionally, P2RX6 is implicated in diverse processes, including immune regulation, inflammation, and neurotransmission. Its expression in immune cells, neurons, and epithelial tissues suggests roles in pathogen defense, pain signaling, and tissue homeostasis. Dysregulation of P2RX6 has been linked to pathologies such as chronic inflammation, neuropathic pain, and certain cancers, though its exact contributions remain understudied. Recombinant P2RX6 enables drug discovery efforts, particularly for targeting ATP-mediated pathways in diseases with purinergic signaling dysfunction. Current research focuses on elucidating its heteromerization dynamics and developing selective modulators to dissect its therapeutic potential.
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