Recombinant Human PCDHa9 protein

**Background of PCDHα9 Recombinant Protein**

Protocadherin alpha 9 (PCDHα9) belongs to the clustered protocadherin (PCDH) family, a subgroup of the cadherin superfamily known for its role in cell-cell adhesion and neural circuit assembly. These transmembrane proteins are characterized by extracellular cadherin repeats that mediate calcium-dependent homophilic interactions and intracellular domains involved in signaling. The PCDHα cluster, located on human chromosome 5. comprises 15 isoforms (α1-α15) with diverse extracellular domains but conserved cytoplasmic regions. PCDHα9. specifically, is implicated in neuronal identity, synaptic specificity, and neural network formation during brain development.

Recombinant PCDHα9 protein is engineered to study its structural and functional properties in vitro. Typically produced in mammalian or bacterial expression systems, the recombinant form retains key domains, such as the extracellular cadherin repeats, enabling research on adhesion mechanics, ligand-receptor interactions, and downstream signaling pathways. Its production often involves affinity tags (e.g., His-tag) for purification and detection.

Research on PCDHα9 is critical in neurodevelopmental and neurodegenerative disorders. Dysregulation of protocadherins has been linked to autism, schizophrenia, and epilepsy, while aberrant PCDH expression is observed in cancers, suggesting roles in tumor suppression or metastasis. Recombinant PCDHα9 facilitates mechanistic studies, antibody development, and high-throughput screening for therapeutic agents. Its application extends to modeling synaptic connectivity and exploring tissue-specific adhesion mechanisms, bridging molecular insights with potential clinical interventions.

In summary, PCDHα9 recombinant protein serves as a vital tool for decoding neural wiring, disease mechanisms, and cadherin-mediated cellular communication.