| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHa13 |
| Uniprot No | Q9Y5I0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-950aa |
| 氨基酸序列 | MLSSWQGGPRPRQLLLWLLILAAWETGSGQLHYSVPEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKRHGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVEVKVRDINDNPPIFPESKKRIIIAESRPPETRFPLDGASDADIGVNSALTYRLDPNDYFTLDAQNSLEQMSSLSLVLRKTLDREEIQEHSLLLTASDGGKPELTGTVQLLITILDVNDNAPEFYQSVYKVTVLENAFNGTLVIKLNATDPDDGTNGDIVYSFRRPVWPAVVYAFTINPNNGEIRTKGKLDFEEKKLYEISVEAVDKGNIPMAGHCTLLVEVLDVNDNAPEVTITSLSLPIREDTQPSAIIALISVSDRDSGSNGQVTCTLTPHVPFKLVSTYKNYYSLVLDSALDRESVSAYELVVTARDGGSPSLWATASVSVGVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSAQDADAQENALVSYSLVERRVGERALSSYVSVHAESGKVYALQPLDHEELELLQFQVSARDSGVPPLGSNVTLQVFVLDENDNAPALLTPGAGSAGGTVSELMPRSVGAGHVVAKVRAVDADSGYNAWLSYELQLAAVGARIPFRVGLYTGEISTTRPLDEVDAPHHRLLVLVKDHGEPALTATATVLLSLVESGQAPQASSRASAGAVGPEAALVDVNVYLIIAICAVSSLLVLTLLLYTALRCSAPPTEGACAPGKPTLVCSSAAGSWSYSQQRRPRVCSGEGPHKTDLMAFSPSLPPCLGSAEGTGQREEDSECLKEPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ |
| 预测分子量 | 102 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于PCDHα13重组蛋白的研究目前公开文献较少，以下为基于相关领域的研究方向提供的参考示例(注：以下为模拟内容，建议通过学术数据库核实具体文献)：
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1. **标题**: "Recombinant PCDHα13 promotes neuronal dendritic arborization via homophilic interactions"
**作者**: Lee, S. et al.
**摘要**: 研究通过昆虫细胞系统表达重组PCDHα13蛋白，发现其通过同源亲和性结合促进神经元树突分支，提示其在神经回路形成中的潜在作用。
2. **标题**: "Expression and purification of PCDHα13 extracellular domain for structural studies"
**作者**: Chen, X. & Wang, Y.
**摘要**: 报道了利用哺乳动物表达系统纯化PCDHα13胞外结构域的方法，并通过质谱和圆二色光谱验证其正确折叠，为后续功能研究奠定基础。
3. **标题**: "PCDHα13 recombinant protein suppresses lung cancer cell migration by modulating Wnt signaling"
**作者**: Gupta, R. et al.
**摘要**: 实验显示，外源性添加重组PCDHα13蛋白可抑制肺癌细胞迁移，机制可能与竞争性结合Wnt通路受体相关，提示其抑癌潜力。
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**建议检索策略**：
- 使用关键词：`PCDHA13 recombinant`、`Protocadherin alpha 13 expression`、`PCDHα13 function`
- 数据库：PubMed、Google Scholar(可筛选近年文献)
- 扩展方向：原钙粘蛋白家族(Protocadherins)在神经发育或癌症中的研究，部分文献可能涉及α亚型重组蛋白应用。
如需具体文献支持，建议进一步结合实验背景补充检索条件。
**Background of PCDHα13 Recombinant Protein**
Protocadherin alpha-13 (PCDHα13) is a member of the protocadherin alpha gene cluster, part of the cadherin superfamily of calcium-dependent cell adhesion molecules. The protocadherin (PCDH) family, particularly the clustered PCDHα, β, and γ subtypes, plays critical roles in neuronal development, including synaptic specificity, axon guidance, and neural circuit assembly. PCDHα13 is one of 15 paralogs in the alpha cluster, characterized by a unique extracellular domain with multiple cadherin-like repeats, a transmembrane domain, and a cytoplasmic tail that interacts with intracellular signaling proteins.
The PCDHα cluster exhibits stochastic monoallelic expression, contributing to neuronal diversity by generating single-cell identity codes. PCDHα13. like other alpha protocadherins, is thought to mediate homophilic interactions, promoting selective adhesion between neurons. This specificity is crucial for establishing precise neural networks during brain development. Dysregulation of PCDHα genes has been linked to neurodevelopmental disorders, psychiatric conditions, and cancer, highlighting their functional importance.
Recombinant PCDHα13 protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional protein for research. It typically includes the extracellular domain to study adhesion mechanisms or full-length protein for signaling analyses. Applications include elucidating PCDHα13's role in neural connectivity, investigating its binding partners, and exploring its involvement in diseases. For instance, abnormal PCDHα13 expression has been observed in certain cancers, suggesting a role in tumor suppression or metastasis.
Studies using recombinant PCDHα13 also aid in developing therapeutic strategies, such as targeting its adhesive properties in neural repair or designing inhibitors for cancer treatment. Overall, PCDHα13 recombinant protein serves as a vital tool for decoding the molecular basis of neural development and disease pathways.
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