| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OPA1 |
| Uniprot No | O60313 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-960aa |
| 氨基酸序列 | MWRLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHLVSRSIYHSHHPTLKL QRPQLRTSFQQFSSLTNLPLRKLKFSPIKYGYQPRRNFWPARLATRLLKL RYLILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWIVPDIVWEIDEYIDFE KIRKALPSSEDLVKLAPDFDKIVESLSLLKDFFTSGSPEETAFRATDRGS ESDKHFRKVSDKEKIDQLQEELLHTQLKYQRILERLEKENKELRKLVLQK DDKGIHHRKLKKSLIDMYSEVLDVLSDYDASYNTQDHLPRVVVVGDQSAG KTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDL TKEEDLAALRHEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMVLVDLP GVINTVTSGMAPDTKETIFSISKAYMQNPNAIILCIQDGSVDAERSIVTD LVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPMKALGYFA VVTGKGNSSESIEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSD CFWKMVRESVEQQADSFKATRFNLETEWKNNYPRLRELDRNELFEKAKNE ILDEVISLSQVTPKHWEEILQQSLWERVSTHVIENIYLPAAQTMNSGTFN TTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFDKL KEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEE ALQARLKDTENAIENMVGPDWKKRWLYWKNRTQEQCVHNETKNELEKMLK CNEEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTAL NHCNLCRRGFYYYQRHFVDSELECNDVVLFWRIQRMLAITANTLRQQLTN TEVRRLEKNVKEVLEDFAEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLD AFIEALHQEK |
| 预测分子量 | 138 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OPA1重组蛋白的3篇参考文献概览：
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1. **文献名称**: *"OPA1 processing controls mitochondrial fusion and is regulated by mRNA splicing, membrane potential, and Yme1L"*
**作者**: Ehses S. et al. (2009)
**摘要**: 该研究阐明了OPA1蛋白通过不同剪切形式(包括重组表达的OPA1变体)调控线粒体内膜融合的机制，发现其活性依赖膜电位，并受蛋白酶Yme1L调控。
2. **文献名称**: *"Structural basis of mitochondrial membrane bending by OPA1"*
**作者**: Faelber K. et al. (2019)
**摘要**: 通过重组OPA1蛋白的体外实验和冷冻电镜分析，揭示了OPA1如何通过寡聚化诱导线粒体膜弯曲，为线粒体嵴形态维持提供结构学证据。
3. **文献名称**: *"Dysfunctional mitochondrial fission and fusion in a patient with OPA1-related optic atrophy"*
**作者**: Amati-Bonneau P. et al. (2008)
**摘要**: 利用患者细胞模型及重组OPA1蛋白回补实验，证明OPA1突变导致线粒体碎片化，重组野生型OPA1可部分恢复融合功能，提示其治疗潜力。
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以上研究覆盖了OPA1重组蛋白在结构、功能机制及疾病治疗中的应用。如需更多文献细节，可进一步检索PubMed或SciHub平台。
**Background of OPA1 Recombinant Protein**
OPA1 (Optic Atrophy 1) is a nuclear-encoded mitochondrial protein critical for maintaining mitochondrial dynamics, membrane integrity, and cellular energy homeostasis. It plays a central role in mitochondrial fusion, cristae organization, and apoptosis regulation. Mutations in the *OPA1* gene are the primary cause of autosomal dominant optic atrophy (ADOA), a neurodegenerative disorder characterized by vision loss due to retinal ganglion cell degeneration. OPA1 exists in multiple isoforms generated through alternative splicing and proteolytic processing, with long isoforms (L-OPA1) anchored to the mitochondrial inner membrane and short isoforms (S-OPA1) promoting mitochondrial fission.
Recombinant OPA1 proteins are engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to study OPA1's structural and functional properties. These proteins enable researchers to investigate mechanisms underlying mitochondrial fusion-fission balance, oxidative phosphorylation, and mitochondrial DNA (mtDNA) maintenance. Recombinant OPA1 is also used to explore therapeutic strategies for diseases linked to mitochondrial dysfunction, such as ADOA, Parkinson’s disease, and cardiomyopathies.
Studies utilizing recombinant OPA1 have revealed its interaction with proteins like MFN2 (involved in outer membrane fusion) and its role in stabilizing mitochondrial cristae during stress. Additionally, OPA1’s cleavage by proteases (e.g., OMA1. YME1L1) under pathological conditions is a focus for understanding mitochondrial fragmentation in aging and disease.
Overall, recombinant OPA1 serves as a vital tool for deciphering mitochondrial biology and developing targeted therapies for mitochondrial disorders. Its application spans basic research, drug screening, and gene therapy approaches aimed at restoring OPA1 function in affected tissues.
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