| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | EXT |
| Uniprot No | Q16394 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-746aa |
| 氨基酸序列 | MQAKKRYFILLSAGSCLALLFYFGGLQFRASRSHSRREEHSGRNGLHHPSPDHFWPRFPDALRPFVPWDQLENEDSSVHISPRQKRDANSSIYKGKKCRMESCFDFTLCKKNGFKVYVYPQQKGEKIAESYQNILAAIEGSRFYTSDPSQACLFVLSLDTLDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFDIGQAMLAKASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGIGSDTRNALYHVHNGEDVVLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFCLVPRGRRLGSFRFLEALQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRSIHQDKILALRQQTQFLWEAYFSSVEKIVLTTLEIIQDRIFKHISRNSLIWNKHPGGLFVLPQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPLVSQSQPVLKLLVAAAKSQYCAQIIVLWNCDKPLPAKHRWPATAVPVVVIEGESKVMSSRFLPYDNIITDAVLSLDEDTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAAIYHKYYHYLYSHYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETMMGQTSRASRWADPDHFAQRQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKYRDIERL |
| 预测分子量 | 86,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EXT重组蛋白的3篇代表性文献示例(内容为模拟概括，实际文献需查证)：
1. **《Functional Analysis of Recombinant EXT1/EXT2 Complex in Heparan Sulfate Biosynthesis》**
- 作者：McCormick C. et al.
- 摘要：研究EXT1和EXT2重组蛋白复合体的体外表达，证实其在硫酸乙酰肝素(HS)链合成中的关键作用，揭示了二者协同催化糖链延伸的分子机制。
2. **《Structural Characterization of Recombinant EXT2 Protein in Hereditary Multiple Osteochondromas》**
- 作者：Saito T. et al.
- 摘要：通过重组EXT2蛋白的晶体结构解析，发现特定突变导致蛋白构象异常，阐明了其与多发性骨软骨瘤发病相关的功能缺陷。
3. **《Recombinant EXT1 Expression in Drosophila Models for Tumor Suppression Studies》**
- 作者：Li H. et al.
- 摘要：利用果蝇模型表达重组EXT1蛋白，证明其通过调控Hedgehog信号通路抑制肿瘤生长，为EXT1的抑癌功能提供体内实验证据。
注：以上为简化示例，实际文献需通过PubMed或专业数据库检索(如关键词：EXT1/EXT2 recombinant protein, exostosin expression)。建议结合具体研究方向筛选近年高引论文。
**Background of EXT Recombinant Proteins**
EXT recombinant proteins are derived from the *EXT1* and *EXT2* genes, which encode critical enzymes involved in heparan sulfate (HS) biosynthesis. HS, a type of glycosaminoglycan (GAG), is a key component of the extracellular matrix and cell surface proteoglycans, playing essential roles in cell signaling, adhesion, and molecular interactions. The EXT1 and EXT2 proteins form a heterocomplex that catalyzes the polymerization of HS chains, a process vital for diverse biological functions, including embryonic development, tissue homeostasis, and growth factor regulation.
Mutations in *EXT1* or *EXT2* are linked to Hereditary Multiple Exostoses (HME), a rare autosomal dominant disorder characterized by benign bone tumors (osteochondromas). This connection underscores the importance of functional EXT proteins in skeletal development. Recombinant EXT proteins are engineered using expression systems (e.g., bacterial, mammalian) to study HS biosynthesis mechanisms, disease pathology, and therapeutic interventions.
The production of recombinant EXT proteins enables researchers to investigate structural and functional abnormalities caused by HME-associated mutations. These proteins also serve as tools for screening potential drugs targeting HS-dependent pathways, such as those involved in cancer, inflammation, and regeneration. Additionally, recombinant EXT variants aid in understanding the broader EXTL gene family, which includes *EXT1*, *EXT2*, and *EXTL1-3*, further elucidating their roles in GAG synthesis and disease.
Overall, EXT recombinant proteins are pivotal in advancing research on HS biology, genetic disorders, and therapeutic development, bridging molecular insights with clinical applications.
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