| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHb8 |
| Uniprot No | Q9UN66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-801aa |
| 氨基酸序列 | MEASGKLICRQRQVLFSFLLLGLSLAGAAEPRSYSVVEETEGSSFVTNLAKDLGLEQREFSRRGVRVVSRGNKLHLQLNQETADLLLNEKLDREDLCGHTEPCVLRFQVLLESPFEFFQAELQVIDINDHSPVFLDKQMLVKVSESSPPGTAFPLKNAEDLDIGQNNIENYIISPNSYFRVLTRKRSDGRKYPELVLDKALDREEEAELRLTLTALDGGSPPRSGTAQVYIEVVDVNDNAPEFEQPFYRVQISEDSPISFLVVKVSATDVDTGVNGEISYSLFQASDEISKTFKVDFLTGEIRLKKQLDFEKFQSYEVNIEARDAGGFSGKCTVLIQVIDVNDHAPEVTMSAFTSPIPENAPETVVALFSVSDLDSGENGKISCSIQEDLPFLLKSSVGNFYTLLTETPLDRESRAEYNVTITVTDLGTPRLTTHLNMTVLVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSLLPPQDPHLPLASLVSINTDNGHLFALRSLDYEALQAFEFRVGASDRGSPALSSEALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKQRLVVLVKDNGEPPCSATATLHVLLVDGFSQPYLPLPEAAPAQGQADSLTVYLVVALASVSSLFLFSVLLFVAVLLCRRSRAASVGRCSVPEGPFPGHLVDVRGTGSLSQNYQYEVCLAGGSGTNEFQLLKPVLPNIQGHSFGPEMEQNSNFRNGFGFSLQLK |
| 预测分子量 | 87,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHb8重组蛋白的3篇参考文献示例(注：部分内容基于假设性研究，实际文献需通过数据库验证)：
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1. **文献名称**: *Expression and Purification of Recombinant Protocadherin-β8 in Mammalian Cells*
**作者**: Li X, et al.
**摘要**: 本研究报道了在HEK293细胞中重组表达人源PCDHb8跨膜蛋白的方法，通过亲和层析纯化获得高纯度蛋白。实验验证了其胞外结构域与神经突触蛋白的相互作用，提示其在神经元黏附中的功能。
2. **文献名称**: *Structural Insights into PCDHb8 Homophilic Binding via Recombinant Ectodomain Analysis*
**作者**: Smith J, et al.
**摘要**: 利用昆虫细胞系统表达PCDHb8胞外域重组蛋白，结合X射线晶体学解析其三维结构，揭示了同源二聚化界面，为原钙粘蛋白家族特异性识别机制提供了证据。
3. **文献名称**: *PCDHb8 Recombinant Protein Modulates Wnt Signaling in Colorectal Cancer Cells*
**作者**: Zhang Y, et al.
**摘要**: 通过大肠杆菌表达体系制备重组PCDHb8胞内段蛋白，发现其过表达可抑制结直肠癌细胞中Wnt/β-catenin通路活性，提示其可能作为肿瘤抑制因子发挥作用。
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**备注**：以上文献为示例性质，实际研究中PCDHb8相关重组蛋白的文献较少，建议通过PubMed或Google Scholar以“PCDHb8 recombinant protein”、“Protocadherin beta 8 expression”等关键词检索最新文献。部分研究可能整合于原钙粘蛋白家族系统性分析中。
Protocadherin-beta-8 (PCDHβ8), a member of the protocadherin beta (PCDHβ) subfamily, is a calcium-dependent cell adhesion molecule predominantly expressed in the vertebrate nervous system. As part of the clustered protocadherin gene cluster, it plays critical roles in neuronal circuit assembly, synaptic specificity, and brain development by mediating homophilic or heterophilic interactions between neurons. The PCDHβ8 protein features a conserved extracellular domain with multiple cadherin repeats, a transmembrane domain, and a variable cytoplasmic tail that may engage in intracellular signaling. Its diversity, arising from alternative splicing and combinatorial expression across the PCDHβ cluster, contributes to the molecular complexity required for neural connectivity.
Recombinant PCDHβ8 protein is typically produced using expression systems like mammalian cells (e.g., HEK293) or insect cells to ensure proper post-translational modifications, particularly glycosylation critical for functional folding. This engineered protein retains the extracellular adhesive domains, enabling in vitro studies on cell-cell interactions, neurite outgrowth, and synaptic plasticity. Researchers utilize it to investigate PCDHβ8's role in neurodevelopmental disorders (e.g., autism, epilepsy) and cancers where protocadherins are often dysregulated. Recent studies also explore its potential as a biomarker or therapeutic target, given its involvement in tumor suppression and metastasis inhibition in certain cancers. Current challenges include elucidating isoform-specific functions and mapping interaction networks within the structurally diverse protocadherin family.
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