| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ATXR4 |
| Uniprot No | Q9FG08 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-325aa |
| 氨基酸序列 | SNRDGDYQIG PPPIRVGLTE SAGRAVFATR KIGAGDLIHT AKPVVACPSL LKLDSVCYLC LKKLMGSAKF EDRGVSYCSQ ECQENSKGFL DVETRADWSS FDDYCRTHNF KYPLMVKRLC CMIISGARPA DCLDILQPAV LSSEMISKIE DGYGLLWNAF RKANFKDDDV AFLTKQWYTA ILARIRINAF RIDLVGGSCG EDLLSLAAAS VEGEGAVGHA VYMLPSFYNH DCDPNAHIIW LHNADARLNT LRDVEEGEEL RICYIDASMG YEARQTILSQ GFGFLCNCLR CQSTD |
| 预测分子量 | 36,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATXR4重组蛋白的3篇参考文献的简要概括(注：ATXR4相关研究较少，以下内容基于现有领域知识模拟，实际文献需通过数据库验证)：
1. **文献名称**: *ATXR4-mediated histone H3K4 methylation regulates Arabidopsis shoot regeneration*
**作者**: Li C. et al.
**摘要**: 该研究通过重组ATXR4蛋白的体外实验，揭示了其在拟南芥茎尖再生过程中催化组蛋白H3K4三甲基化的作用，证明其通过表观遗传调控干细胞多能性基因的表达。
2. **文献名称**: *Biochemical characterization of recombinant ATXR4 in chromatin remodeling*
**作者**: Zhang Y. et al.
**摘要**: 报道了ATXR4重组蛋白的纯化及酶活分析，证实其具有特异性H3K4甲基转移酶活性，并发现其与染色质重塑复合体CLF-PRC2存在功能互作。
3. **文献名称**: *ATXR4 stabilizes genome integrity by regulating replication fork progression*
**作者**: Wang H. et al.
**摘要**: 利用重组ATXR4蛋白进行体外DNA结合实验，发现其通过调控复制叉进程维持基因组稳定性，依赖其N端结构域与染色质的相互作用。
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**说明**：上述内容为领域知识模拟，实际研究中ATXR4(拟南芥Trithorax相关蛋白4)的研究多集中于表观遗传调控和发育生物学。建议通过PubMed或Google Scholar以“ATXR4 recombinant”或“ATXR4 histone methylation”为关键词检索最新文献。
ATXR4 (Arabidopsis Trithorax-Related 4) is a plant-specific histone methyltransferase implicated in epigenetic regulation. As a member of the Trithorax group (TrxG) proteins, it plays a role in maintaining active gene expression by depositing histone H3 lysine 4 monomethylation (H3K4me1), a chromatin modification associated with transcriptional activation. First characterized in *Arabidopsis thaliana*, ATXR4 is distinct from its paralogs (e.g., ATXR3/5/6) due to its unique substrate specificity and functional specialization. Structurally, it contains a conserved SET domain responsible for catalytic activity and a divergent N-terminal region that may mediate protein interactions or subcellular localization.
Studies suggest ATXR4 regulates developmental processes, including flowering time and leaf morphogenesis, by modulating chromatin states at specific loci. Its activity counteracts Polycomb Repressive Complex 2 (PRC2)-mediated H3K27me3. balancing gene activation and repression. Recombinant ATXR4 protein, produced via heterologous expression systems (e.g., *E. coli* or insect cells), enables biochemical studies to dissect its enzymatic mechanisms, substrate preferences, and interaction partners. Such research has revealed its dependence on S-adenosylmethionine (SAM) as a methyl donor and its preferential activity toward nucleosomal substrates over free histones.
The generation of recombinant ATXR4 has also facilitated structural analyses and high-throughput screening for small-molecule inhibitors or activators, with potential applications in plant biotechnology or epigenetic drug discovery. However, functional redundancy with other TrxG members and tissue-specific roles remain under investigation. Current work aims to map its genome-wide targets and explore its interplay with other chromatin modifiers, providing insights into conserved epigenetic pathways across eukaryotes. ATXR4 exemplifies how plant-specific chromatin regulators can offer unique models to study gene expression control.
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