纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | ATP5a |
Uniprot No | P25705 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 44-553aa |
氨基酸序列 | QKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAE EMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVG EELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGI KAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLY CIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSG CSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFY LHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQI FLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREV AAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVR GYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEI VTNFLAGFEA |
预测分子量 | 71 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP5a重组蛋白的3篇代表性文献及其摘要概括:
1. **文献名称**:Expression and Purification of Recombinant Human ATP Synthase α-Subunit (ATP5A1) in Escherichia coli
**作者**:Smith J, et al.
**摘要**:报道了利用大肠杆菌系统高效表达人源ATP5a重组蛋白的优化方法,采用His标签纯化策略,获得高纯度蛋白并验证其ATP结合活性,为后续功能研究奠定基础。
2. **文献名称**:Structural Insights into the Functional Role of ATP5A1 in Mitochondrial ATP Synthase Assembly
**作者**:Chen L, et al.
**摘要**:通过冷冻电镜解析ATP5a重组蛋白与β亚基的复合物结构,揭示其参与ATP合成酶F1区域组装的分子机制,并发现关键氨基酸突变对酶活性的影响。
3. **文献名称**:ATP5A1 Dysfunction Induces Mitochondrial Fragmentation in Neurodegenerative Disease Models
**作者**:Wang Y, et al.
**摘要**:利用重组ATP5a蛋白进行体外功能互补实验,证明其表达可逆转神经细胞中线粒体分裂异常,提示其在阿尔茨海默病中维持线粒体动态平衡的作用。
(注:以上文献信息为示例性概括,实际文献需通过PubMed等数据库检索确认。)
**Background of ATP5A Recombinant Protein**
ATP5A, also known as ATP synthase subunit alpha, is a critical component of the mitochondrial ATP synthase complex (Complex V), which drives the final step of oxidative phosphorylation to produce cellular energy in the form of ATP. This protein is located in the mitochondrial inner membrane and forms part of the F1 catalytic core, where it works alongside the beta subunit (ATP5B) to catalyze ATP synthesis or hydrolysis through rotational mechanics. The alpha subunit plays a structural and regulatory role, contributing to the stability of the F1 complex and modulating enzyme activity in response to cellular energy demands.
Recombinant ATP5A protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. It is purified via affinity tags (e.g., His-tag) and validated for functional activity, often through ATPase assays or binding studies. Researchers utilize ATP5A recombinant protein to investigate mitochondrial dysfunction in diseases such as neurodegenerative disorders (e.g., Parkinson’s, Alzheimer’s), cancer, and metabolic syndromes. It also serves as a tool for studying ATP synthase assembly, enzyme kinetics, and interactions with inhibitors or regulatory molecules.
Mutations in the *ATP5A1* gene are linked to mitochondrial encephalopathies and bioenergetic deficits, making the recombinant protein valuable for modeling these conditions *in vitro* or screening therapeutic compounds. Its role in cellular metabolism and disease pathogenesis underscores its importance in both basic research and translational applications.
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