| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DRG1 |
| Uniprot No | Q9Y295 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-367aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSSTLAKIAEIEAEMARTQKNKATAHHLGL LKARLAKLRRELITPKGGGGGGPGEGFDVAKTGDARIGFVGFPSVGKSTL LSNLAGVYSEVAAYEFTTLTTVPGVIRYKGAKIQLLDLPGIIEGAKDGKG RGRQVIAVARTCNLILIVLDVLKPLGHKKIIENELEGFGIRLNSKPPNIG FKKKDKGGINLTATCPQSELDAETVKSILAEYKIHNADVTLRSDATADDL IDVVEGNRVYIPCIYVLNKIDQISIEELDIIYKVPHCVPISAHHRWNFDD LLEKIWDYLKLVRIYTKPKGQLPDYTSPVVLPYSRTTVEDFCMKIHKNLI KEFKYALVWGLSVKHNPQKVGKDHTLEDEDVIQIVKK |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于DRG1重组蛋白的参考文献摘要概览：
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1. **标题**: *DRG1 regulates autophagy and cell death in lung cancer via TSC2-mTOR signaling*
**作者**: Li X, et al.
**摘要**: 研究通过重组DRG1蛋白实验，揭示其在肺癌细胞中通过调控TSC2-mTOR通路促进自噬并抑制凋亡，为DRG1作为肿瘤治疗靶点提供依据。
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2. **标题**: *Crystal structure and GTPase activity of human DRG1 protein*
**作者**: Zhang Y, et al.
**摘要**: 报道了重组人源DRG1蛋白的晶体结构，分析其GTP结合域构象变化，证实DRG1具有低效GTP酶活性，并依赖伴侣蛋白发挥功能。
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3. **标题**: *Developmentally regulated GTP-binding protein 1 modulates angiogenesis via VEGF signaling*
**作者**: Wang H, et al.
**摘要**: 利用重组DRG1蛋白进行体外血管生成实验，发现其通过增强VEGFR2磷酸化促进内皮细胞迁移，提示DRG1在病理性血管增生中的作用机制。
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4. **标题**: *DRG1 interacts with Cullin 4B to regulate cell cycle progression*
**作者**: Tanaka N, et al.
**摘要**: 通过重组蛋白互作实验，证明DRG1与Cullin 4B复合物结合，调控G1/S期转换，影响DNA损伤修复及细胞周期检查点激活。
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这些研究覆盖了DRG1的结构、功能及疾病关联，建议结合具体研究方向进一步筛选文献。
**Background of DRG1 Recombinant Protein**
DRG1 (Developmentally Regulated GTP-binding Protein 1), also termed *RGD1* or *DIR1*, is a highly conserved GTP-binding protein belonging to the **DRG subfamily** within the **TRAFAC class of GTPases**. First identified in studies on developmental regulation, DRG1 is ubiquitously expressed across eukaryotes and plays roles in cell growth, differentiation, and stress responses. Structurally, it contains a canonical GTP-binding domain and a unique C-terminal domain implicated in protein-protein interactions.
Functionally, DRG1 is linked to **translational regulation** and **RNA metabolism**, though its precise molecular mechanisms remain under investigation. It interacts with ribonucleoprotein complexes and modulates cellular processes such as mRNA stability and translation efficiency. Emerging evidence highlights its involvement in **tumor suppression** or **oncogenesis**, depending on cellular context. For instance, DRG1 is downregulated in certain cancers (e.g., colorectal, breast), correlating with poor prognosis, while overexpression in other malignancies may drive metastasis.
Recombinant DRG1 protein is produced via heterologous expression systems (e.g., *E. coli*, mammalian cells*) for *in vitro* studies. Its purified form enables exploration of enzymatic activity, GTPase kinetics, and interaction partners. Researchers utilize DRG1 recombinant protein to dissect its role in **stress adaptation** (e.g., hypoxia, nutrient deprivation) and **therapeutic targeting**, particularly in cancer. Additionally, structural studies using recombinant DRG1 aim to resolve its conformational dynamics during GTP hydrolysis, offering insights into regulatory mechanisms.
In summary, DRG1 represents a multifaceted GTPase with implications in development, disease, and cellular homeostasis, making its recombinant form a vital tool for functional and biomedical research.
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