| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPY30 |
| Uniprot No | Q9C005 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-99aa |
| 氨基酸序列 | EPEQMLEGQTQVAENPHSEYGLTDNVERIVENEKINAEKSSKQKVDLQSL PTRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPY30重组蛋白的3篇代表性文献的简要概括：
1. **文献名称**：DPY30 regulates canonical Wnt signaling via the interaction with EED or through its role in the COMPASS complex
**作者**：Yang Z, et al.
**摘要**：该研究通过重组DPY30蛋白，揭示了其通过与EED蛋白互作或作为COMPASS复合物亚基调控Wnt/β-catenin信号通路的双重机制，影响胚胎干细胞分化。
2. **文献名称**：Structural basis of DPY30 homodimer-mediated COMPASS assembly in H3K4 methylation
**作者**：Xiao B, et al.
**摘要**：通过X射线晶体学解析重组DPY30同源二聚体结构，阐明了其作为支架蛋白协调COMPASS复合物亚基组装，促进组蛋白H3K4三甲基化的分子机制。
3. **文献名称**：DPY30 interacts with the histone variant H2A.Z to modulate cellular differentiation
**作者**：Chen Y, et al.
**摘要**：利用重组DPY30进行蛋白质互作筛选，发现其与组蛋白变体H2A.Z存在功能协同，共同调控基因启动子区域的染色质重塑和细胞命运决定。
注：以上文献为模拟示例，实际文献需通过PubMed或Web of Science检索关键词"DPY30 recombinant protein"获取。建议优先选择Nature Structural & Molecular Biology、Cell Reports等期刊近5年发表的研究论文。
DPY30 is a conserved regulatory subunit of the Set1/MLL (Mixed-Lineage Leukemia) family of histone methyltransferase complexes, which catalyze the trimethylation of histone H3 at lysine 4 (H3K4me3), a hallmark of transcriptionally active chromatin. It also interacts with the Polycomb Repressive Complex 2 (PRC2), which mediates H3K27 trimethylation (H3K27me3) associated with gene silencing. This dual association positions DPY30 as a critical modulator of epigenetic balance, influencing chromatin dynamics and gene expression programs during development and cellular differentiation.
The recombinant DPY30 protein is engineered to enable precise biochemical and functional studies. Typically produced in bacterial or mammalian expression systems, recombinant DPY30 retains its ability to bind core components of both Set1/MLL and PRC2 complexes. Structural studies reveal that DPY30 contains an N-terminal domain essential for complex assembly and a C-terminal region involved in stabilizing interactions with other subunits. Its small size (~10 kDa) and conserved structure across eukaryotes make it a model for studying epigenetic regulation mechanisms.
Research using recombinant DPY30 has elucidated its role in enhancing the catalytic activity of Set1/MLL complexes, ensuring proper H3K4me3 deposition. Loss of DPY30 disrupts embryonic stem cell pluripotency and somatic cell reprogramming, highlighting its importance in cell fate determination. Aberrant DPY30 expression has been linked to cancers and neurodevelopmental disorders, underscoring its therapeutic potential. Recombinant forms are pivotal for drug screening, structural analysis, and dissecting crosstalk between activating and repressive chromatin modifiers.
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