| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BNC2 |
| Uniprot No | Q6ZN30 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1099aa |
| 氨基酸序列 | MAHLGPTPPPHSLNYKSEDRLSEQDWPAYFKVPCCGVDTSQIESEEAEVDVRERETQRDREPKRARDLTLRDSCTDNSMQFGTRTTTAEPGFMGTWQNADTNLLFRMSQQAIRCTLVNCTCECFQPGKINLRTCDQCKHGWVAHALDKLSTQHLYHPTQVEIVQSNVVFDISSLMLYGTQAVPVRLKILLDRLFSVLKQEEVLHILHGLGWTLRDYVRGYILQDAAGKVLDRWAIMSREEEIITLQQFLRFGETKSIVELMAIQEKEGQAVAVPSSKTDSDIRTFIESNNRTRSPSLLAHLENSNPSSIHHFENIPNSLAFLLPFQYINPVSAPLLGLPPNGLLLEQPGLRLREPSLSTQNEYNESSESEVSPTPYKNDQTPNRNALTSITNVEPKTEPACVSPIQNSAPVSDLTKTEHPKSSFRIHRMRRMGSASRKGRVFCNACGKTFYDKGTLKIHYNAVHLKIKHRCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMLRNNRDKDLIRATSGAATPVIASTKSNLALTSPGRPPMGFTTPPLDPVLQNPLPSQLVFSGLKTVQPVPPFYRSLLTPGEMVSPPTSLPTSPIIPTSGTIEQHPPPPSEPVVPAVMMATHEPSADLAPKKKPRKSSMPVKIEKEIIDTADEFDDEDDDPNDGGAVVNDMSHDNHCHSQEEMSPGMSVKDFSKHNRTRCISRTEIRRADSMTSEDQEPERDYENESESSEPKLGEESMEGDEHIHSEVSEKVLMNSERPDENHSEPSHQDVIKVKEEFTDPTYDMFYMSQYGLYNGGGASMAALHESFTSSLNYGSPQKFSPEGDLCSSPDPKICYVCKKSFKSSYSVKLHYRNVHLKEMHVCTVAGCNAAFPSRRSRDRHSANINLHRKLLTKELDDMGLDSSQPSLSKDLRDEFLVKIYGAQHPMGLDVREDASSPAGTEDSHLNGYGRGMAEDYMVLDLSTTSSLQSSSSIHSSRESDAGSDEGILLDDIDGASDSGESAHKAEAPALPGSLGAEVSGSLMFSSLSGSNGGIMCNICHKMYSNKGTLRVHYKTVHLREMHKCKVPGCNMMFSSVRSRNRHSQNPNLHKNIPFTSVD |
| 预测分子量 | 122 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于BNC2重组蛋白的相关文献概览：
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1. **"BNC2 regulates cell proliferation in epithelial ovarian cancer via the TGF-β signaling pathway"**
- **作者**: Li et al. (2020)
- **摘要**: 本研究通过重组BNC2蛋白体外表达，发现其通过调控TGF-β信号通路抑制卵巢癌细胞增殖。实验表明，BNC2过表达显著降低细胞周期相关蛋白水平，提示其作为肿瘤抑制因子的潜在作用。
2. **"Recombinant BNC2 protein inhibits melanoma metastasis by modulating integrin-mediated adhesion"**
- **作者**: Smith & Johnson (2018)
- **摘要**: 该文献利用大肠杆菌系统表达纯化的BNC2重组蛋白，发现其能阻断黑色素瘤细胞的整合素依赖性黏附和迁移，揭示BNC2在抑制肿瘤转移中的分子机制。
3. **"Structural and functional analysis of Basonuclin-2 (BNC2) zinc finger domains"**
- **作者**: Garcia et al. (2017)
- **摘要**: 通过重组BNC2蛋白的锌指结构域体外表达，解析其DNA结合特性。实验证实BNC2通过特定锌指基序与靶基因启动子结合，调控皮肤成纤维细胞的分化过程。
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注：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science检索最新具体文献。BNC2相关研究多集中于其在癌症、纤维化及发育调控中的作用，重组蛋白技术常被用于功能验证和机制研究。
**Background of BNC2 Recombinant Protein**
BNC2 (Basonuclin-2) is a zinc finger-containing protein encoded by the *BNC2* gene, primarily involved in transcriptional regulation and cellular processes such as differentiation and proliferation. It shares structural homology with Basonuclin-1 (BNC1), characterized by conserved zinc finger domains that mediate DNA binding. BNC2 is expressed in various tissues, including the skin, reproductive organs, and developing skeletal systems, and has been implicated in developmental biology, tissue homeostasis, and disease pathways.
Recombinant BNC2 protein is engineered using biotechnological methods (e.g., *E. coli* or mammalian expression systems) to produce purified, functional BNC2 for research applications. Its recombinant form enables studies on molecular interactions, signaling mechanisms, and structural analyses. Interest in BNC2 stems from its roles in diseases such as melanoma, ovarian cancer, and fibrosis, where dysregulated expression correlates with tumor progression or tissue remodeling. For instance, BNC2 acts as a tumor suppressor in some cancers by modulating pathways like TGF-β signaling, while also influencing extracellular matrix dynamics in fibrotic disorders.
Structurally, BNC2 contains N-terminal transactivation domains and C-terminal zinc finger motifs, which are critical for DNA binding and transcriptional activity. Recombinant protein studies help dissect these functional domains and their interactions with co-regulators or DNA targets. Additionally, BNC2 polymorphisms are linked to human traits, including hair morphology and cancer susceptibility, making it a focus for genetic and pharmacogenetic research.
Overall, BNC2 recombinant protein serves as a vital tool for elucidating its biological roles and exploring therapeutic strategies targeting its pathways in development and disease.
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