| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRIN3B |
| Uniprot No | O60391 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1043aa |
| 氨基酸序列 | MEFVRALWLGLALALGPGSAGGHPQPCGVLARLGGSVRLGALLPRAPLARARARAALARAALAPRLPHNLSLELVVAAPPARDPASLTRGLCQALVPPGVAALLAFPEARPELLQLHFLAAATETPVLSLLRREARAPLGAPNPFHLQLHWASPLETLLDVLVAVLQAHAWEDVGLALCRTQDPGGLVALWTSRAGRPPQLVLDLSRRDTGDAGLRARLAPMAAPVGGEAPVPAAVLLGCDIARARRVLEAVPPGPHWLLGTPLPPKALPTAGLPPGLLALGEVARPPLEAAIHDIVQLVARALGSAAQVQPKRALLPAPVNCGDLQPAGPESPGRFLARFLANTSFQGRTGPVWVTGSSQVHMSRHFKVWSLRRDPRGAPAWATVGSWRDGQLDLEPGGASARPPPPQGAQVWPKLRVVTLLEHPFVFARDPDEDGQCPAGQLCLDPGTNDSATLDALFAALANGSAPRALRKCCYGYCIDLLERLAEDTPFDFELYLVGDGKYGALRDGRWTGLVGDLLAGRAHMAVTSFSINSARSQVVDFTSPFFSTSLGIMVRARDTASPIGAFMWPLHWSTWLGVFAALHLTALFLTVYEWRSPYGLTPRGRNRSTVFSYSSALNLCYAILFRRTVSSKTPKCPTGRLLMNLWAIFCLLVLSSYTANLAAVMVGDKTFEELSGIHDPKLHHPAQGFRFGTVWESSAEAYIKKSFPDMHAHMRRHSAPTTPRGVAMLTSDPPKLNAFIMDKSLLDYEVSIDADCKLLTVGKPFAIEGYGIGLPQNSPLTSNLSEFISRYKSSGFIDLLHDKWYKMVPCGKRVFAVTETLQMSIYHFAGLFVLLCLGLGSALLSSLGEHAFFRLALPRIRKGSRLQYWLHTSQKIHRALNTEPPEGSKEETAEAEPSGPEVEQQQQQQDQPTAPEGWKRARRAVDKERRVRFLLEPAVVVAPEADAEAEAAPREGPVWLCSYGRPPAARPTGAPQPGELQELERRIEVARERLRQALVRRGQLLAQLGDSARHRPRRLLQARAAPAEAPPHSGRPGSQE |
| 预测分子量 | 112 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GRIN3B重组蛋白的模拟参考文献示例(请注意，以下内容为示例，非真实文献)：
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1. **文献名称**: "Recombinant GRIN3B Subunit Expression and Functional Characterization in NMDA Receptors"
**作者**: Smith A, et al.
**摘要**: 研究通过哺乳动物细胞表达系统成功重组表达GRIN3B蛋白，并验证其与GRIN1亚基共表达时形成功能性NMDA受体。研究揭示了GRIN3B对受体电流动力学的调控作用，为研究NMDA受体异质性提供工具。
2. **文献名称**: "Structural Insights into the GRIN3B Subunit via Cryo-EM Analysis"
**作者**: Chen L, et al.
**摘要**: 利用冷冻电镜技术解析了含GRIN3B的NMDA受体复合物结构，首次揭示其跨膜结构域和配体结合位点的构象差异，为靶向神经精神疾病的药物设计提供结构基础。
3. **文献名称**: "GRIN3B Polymorphisms and Recombinant Protein Applications in Schizophrenia Models"
**作者**: Kim H, et al.
**摘要**: 探讨GRIN3B基因变异与精神分裂症的相关性，通过重组GRIN3B蛋白体外实验证明其异常表达可能影响突触可塑性，提示其在疾病病理机制中的潜在作用。
4. **文献名称**: "Optimization of GRIN3B Recombinant Production in E. coli for Biochemical Studies"
**作者**: Patel R, et al.
**摘要**: 报道了一种通过大肠杆菌高效表达GRIN3B重组蛋白的优化方案，并验证其与天然蛋白的抗原性和功能相似性，为大规模制备及抗体开发提供技术支持。
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**备注**：以上文献为模拟示例，实际研究中建议通过PubMed或Google Scholar以关键词“GRIN3B recombinant protein”或“NMDA receptor GRIN3B”检索最新文献。真实研究可能集中于NMDA受体复合物功能、神经疾病机制或重组蛋白表达技术等领域。
**Background of GRIN3B Recombinant Protein**
GRIN3B (glutamate ionotropic receptor NMDA type subunit 3B) is a key component of N-methyl-D-aspartate (NMDA) receptors, a subclass of ionotropic glutamate receptors critical for synaptic plasticity, learning, and memory. Encoded by the *GRIN3B* gene, this subunit is distinct within the NMDA receptor family due to its unique functional and structural properties. Unlike other NMDA receptor subunits (e.g., GRIN1. GRIN2A-D), GRIN3B requires co-assembly with a GRIN1 subunit to form functional receptors. These receptors are ligand-gated cation channels activated by the simultaneous binding of glutamate and glycine/D-serine, facilitating calcium influx and modulating neuronal signaling.
GRIN3B-containing receptors exhibit distinct biophysical and pharmacological traits, such as reduced magnesium sensitivity and altered channel kinetics, which diversify NMDA receptor signaling in the central nervous system. While GRIN3B is expressed at lower levels compared to other subunits, it is enriched in specific brain regions (e.g., thalamus, hippocampus) and peripheral tissues, suggesting roles beyond classical synaptic transmission, including neurodevelopment, pain processing, and neuropsychiatric disorders.
Recombinant GRIN3B protein is engineered using expression systems (e.g., mammalian cells, *E. coli*) to produce purified, functional subunits for research. This tool enables studies on receptor assembly, ligand interactions, and downstream signaling mechanisms. Dysregulation of GRIN3B has been linked to neurological conditions such as schizophrenia, depression, and Alzheimer’s disease, making it a potential therapeutic target. Recombinant GRIN3B also aids in drug screening to identify modulators of NMDA receptor activity, offering insights into treating disorders associated with glutamate signaling imbalances.
In summary, GRIN3B recombinant protein serves as a vital resource for unraveling the complexity of NMDA receptor biology and advancing translational research in brain disorders.
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