| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FUT2 |
| Uniprot No | Q10981 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343aa |
| 氨基酸序列 | MLVVQMPFSFPMAHFILFVFTVSTIFHVQQRLAKIQAMWELPVQIPVLASTSKALGPSQLRGMWTINAIGRLGNQMGEYATLYALAKMNGRPAFIPAQMHSTLAPIFRITLPVLHSATASRIPWQNYHLNDWMEEEYRHIPGEYVRFTGYPCSWTFYHHLRQEILQEFTLHDHVREEAQKFLRGLQVNGSRPGTFVGVHVRRGDYVHVMPKVWKGVVADRRYLQQALDWFRARYSSLIFVVTSNGMAWCRENIDTSHGDVVFAGDGIEGSPAKDFALLTQCNHTIMTIGTFGIWAAYLTGGDTIYLANYTLPDSPFLKIFKPEAAFLPEWTGIAADLSPLLKH |
| 预测分子量 | 39,0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FUT2重组蛋白的3篇代表性文献示例(内容为模拟概括，非真实文献)：
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1. **文献名称**: *Expression and functional characterization of recombinant human FUT2 enzyme in Escherichia coli*
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了His标签的FUT2重组蛋白，优化了诱导条件(如IPTG浓度和温度)，并通过质谱和SDS-PAGE验证蛋白正确性。功能实验证实该重组蛋白具有α-1.2-岩藻糖转移酶活性，可催化H抗原合成，为后续研究提供可靠工具。
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2. **文献名称**: *Structural basis of substrate specificity in FUT2-mediated fucosylation*
**作者**: Suzuki K, et al.
**摘要**: 文章解析了重组FUT2蛋白的晶体结构，结合分子动力学模拟阐明了其底物结合口袋的关键氨基酸残基(如Arg148和Asp226)。实验表明FUT2优先识别Ⅰ型糖链(Galβ1-3GlcNAc)，而非Ⅱ型结构，揭示了其在肠道黏液素糖基化中的选择性作用。
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3. **文献名称**: *Recombinant FUT2 modulates gut microbiota composition and reduces susceptibility to rotavirus infection in vitro*
**作者**: Kelly RJ, et al.
**摘要**: 利用哺乳动物细胞表达的重组FUT2蛋白处理肠道上皮细胞，发现其通过增加表面ABH抗原表达，抑制轮状病毒结合(降低感染率50%以上)。同时，16S rRNA测序显示FUT2重组蛋白处理改变了宿主-微生物互作，促进双歧杆菌等有益菌定植。
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**注**：以上文献为示例性概括，实际研究需通过PubMed/Google Scholar检索关键词(如“FUT2 recombinant protein expression”或“FUT2 enzymatic activity”)获取真实文献。如需具体论文，可进一步提供数据库检索指导。
**Background of FUT2 Recombinant Protein**
FUT2 (Fucosyltransferase 2) is a key enzyme encoded by the *FUT2* gene, responsible for transferring fucose residues to glycan chains, a process critical for synthesizing histo-blood group antigens (HBGAs) and regulating mucosal glycosylation. It plays a pivotal role in shaping the composition of gut microbiota by producing soluble ABO antigens in bodily secretions (e.g., saliva, intestinal mucus), earning individuals with active FUT2 as "secretors" (~80% of populations) and those with non-functional variants as "non-secretors." This secretory status impacts susceptibility to infections, immune responses, and chronic diseases, including inflammatory bowel disease (IBD) and metabolic disorders.
Recombinant FUT2 protein is engineered using biotechnological methods, where the *FUT2* gene is cloned into expression vectors (e.g., bacterial, mammalian, or insect cell systems) to produce purified, functional enzyme. This allows precise study of its enzymatic activity, substrate specificity, and interactions with microbial pathogens (e.g., norovirus, *Helicobacter pylori*) that exploit HBGAs for host adhesion. Researchers also utilize FUT2 recombinant protein to investigate glycan-mediated host-microbe crosstalk, develop glycan-based therapeutics, or model diseases linked to aberrant fucosylation.
Its applications extend to diagnostics, vaccine development, and personalized medicine, particularly in understanding how genetic FUT2 variations influence disease risks and microbiome dynamics. Studies on recombinant FUT2 continue to unravel its dual role in promoting microbial symbiosis and predisposing to specific pathologies, highlighting its potential as a therapeutic target or biomarker.
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