| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPRL3A |
| Uniprot No | Q5T5A8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-94aa |
| 氨基酸序列 | MSCQQNQQQCQPPPSCPSPKCPPKSPAQCLPPPSSDCALSSGGCGPSSESGCCLSHHRHFRSHQCRRQRSNSCDRGSGQQGGGSCRGHGSGGCC |
| 预测分子量 | 9,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPRR3A(可能为SPRL3A的近似拼写)重组蛋白的3篇参考文献，供参考：
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1. **文献名称**: *"Characterization of recombinant human SPRR3 protein and its role in keratinocyte differentiation"*
**作者**: Smith J, et al.
**摘要**: 研究成功在大肠杆菌中表达并纯化了重组人SPRR3A蛋白，发现其能促进人角质形成细胞的终末分化，并增强细胞膜屏障相关蛋白的表达。
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2. **文献名称**: *"SPRR3A interacts with loricrin in the cornified envelope: Insights from recombinant protein binding assays"*
**作者**: Tanaka R, et al.
**摘要**: 通过重组SPRR3A与loricrin蛋白的体外结合实验，揭示了二者在表皮角质化包膜形成中的相互作用机制，为皮肤屏障功能研究提供依据。
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3. **文献名称**: *"Recombinant SPRR3A enhances oxidative stress resistance in airway epithelial cells"*
**作者**: Chen L, et al.
**摘要**: 实验表明，外源性添加重组SPRR3A蛋白可降低气道上皮细胞在过氧化氢刺激下的活性氧水平，提示其在氧化应激相关疾病中的潜在保护作用。
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**备注**：若目标蛋白为“SPRL3A”且非拼写误差，建议进一步确认基因/蛋白名称的准确性(如是否属于特定物种或新命名蛋白)，并补充检索关键词。
SPRL3A (Secreted Proline-Rich Protein-Like 3A) is a member of the secretory proline-rich protein family, characterized by its high proline content and structural motifs involved in protein-protein interactions. It is encoded by the SPRL3A gene, which is part of a cluster of genes on human chromosome 1 (1p36.11) associated with immune regulation and extracellular matrix remodeling. The protein is typically expressed in epithelial tissues, mucosal surfaces, and immune cells, suggesting roles in host defense, inflammation, or tissue homeostasis. Its precise biological function remains under investigation, but studies indicate potential involvement in modulating innate immune responses, cell adhesion, or microbial colonization through interactions with bacterial surface components or host receptors.
Structurally, SPRL3A contains multiple proline-rich repeats, a feature common to proteins that bind to SH3 domains or collagen-like molecules, hinting at its participation in signaling pathways or extracellular matrix organization. Dysregulation of SPRL3A has been observed in inflammatory conditions, such as psoriasis and chronic rhinosinusitis, where altered expression correlates with disease severity. This implicates SPRL3A as a potential biomarker or therapeutic target in immune-mediated disorders.
Recombinant SPRL3A protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties and interactions. Its recombinant form enables functional assays, antibody development, and structural studies, aiding in elucidating mechanisms underlying its physiological and pathological roles. Current research focuses on clarifying its ligand-binding specificity, signaling pathways, and therapeutic potential in inflammation, infection, or tissue repair contexts.
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