| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHgA3 |
| Uniprot No | Q9Y5H0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-932aa |
| 氨基酸序列 | MTNCLSFRNGRGLALLCALLGTLCETGSGQIRYSVSEELDKGSFVGNIANDLGLEPRELAERGVRIVSRGRTQLFSLNPQSGSLVTAERIDREELCAQIPLCLVKINILVEDKLKIFEVEIEIKDINDNAPNFPTEELEIKIGELTVPGTRFPIKTAFDPDVGINSLQNYKLSPNDYFSLAVNSVSEGAKYPELVLERALDREKKEIHQLVLVASDGGDPVHSGNLHIQVIVLDANDNPPMFTQPEYRVSVWENVPVGTRLLTVNATDPDEGFNAQVSYILDKMPGKIAEIFHLNSVSGEVSILKSLDYEDAMFYEIKIEAQDGPGLLSRAKILVTVLDVNDNAPEITITSLTSSVPEEGTVGREIALIDVHDRDSGQNGQVEVFVLGNLPFKLEKSIDQYYRLVTATSLDREQISEYNISLRASDGGSPPLSTETHITLHVIDINDNPPTFPHLSYSAYIPENNPRGASIFSVTAQDPDSNNNARITYALTEDTLQGAPLSSFVSINSNTGVLYALRSFDYEQFRDLKLLVTASDSGNPPLSSNVSLNLFVLDQNDNAPEILYPALPTDGSTGVELAPRSAEPGYLVTKVVAVDRDSGQNAWLSYRLLKASEPGLFSVGLHTGEVRTARALLDRDALKQSLVVAVQDHGQPPLSATVTLTVAVADRIPDILADLGSLEPSAKPNDSDLTLYLVVAVAAVSCVFLAFVIVLLALRLRRWHKSRLLQASGGGLASTPGSHFVGADGVRAFLQTYSHEVSLTADSRKSHLIFPQPNYADTLISQESCEKSEPLLITQDLLEMKGDSNLLQQAPPNTDWRFSQAQRPGTSGSQNGDDTGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK |
| 预测分子量 | 100,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHgA3重组蛋白的模拟参考文献示例(注：以下内容为示例性构造，非真实存在的文献)：
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1. **《Recombinant Expression and Functional Characterization of Protocadherin-gamma A3 in Neuronal Adhesion》**
- **作者**: Li, X.; Zhang, Y.; Chen, J.
- **摘要**: 本研究成功在HEK293细胞中表达了重组PCDHgA3蛋白，并验证其通过同源相互作用介导的细胞粘附活性。实验表明，PCDHgA3的胞外结构域在神经元突触形成中可能起关键作用。
2. **《Structural Analysis of PCDHgA3 Recombinant Protein Using Cryo-EM》**
- **作者**: Smith, A.R.; Johnson, T.K.
- **摘要**: 通过冷冻电镜解析了重组PCDHgA3蛋白的胞外结构，揭示了其独特的二聚化模式及钙离子结合位点，为理解原钙粘蛋白家族的结构-功能关系提供了新见解。
3. **《Optimization of PCDHgA3 Expression in E. coli for High-Yield Production》**
- **作者**: Wang, H.; Kim, S.; Gupta, R.
- **摘要**: 报道了一种在大肠杆菌系统中高效表达可溶性PCDHgA3重组蛋白的方法，通过密码子优化和亲和层析纯化获得高纯度蛋白，适用于抗体开发及体外功能研究。
4. **《PCDHgA3 Recombinant Protein Modulates Synaptic Plasticity in Cortical Neurons》**
- **作者**: Martinez, E.; Lee, D.H.
- **摘要**: 使用重组PCDHgA3蛋白处理原代神经元，发现其通过激活下游信号通路增强突触可塑性，提示其在神经发育疾病中的潜在治疗价值。
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**注**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。如需进一步协助定位真实文献，建议提供更具体的研究方向或实验背景。
**Background of PCDHgA3 Recombinant Protein**
The protocadherin gamma subfamily A3 (PCDHgA3) is a member of the clustered protocadherin (PCDH) family, a group of transmembrane cell adhesion molecules critical for neural development, synaptic specificity, and neuronal connectivity. Protocadherins are subdivided into α, β, and γ clusters, with the γ cluster further divided into A and B subfamilies. PCDHgA3. encoded by the *PCDHGA3* gene, is part of the γ-A subfamily, which comprises 12 highly homologous isoforms (A1–A12) generated through alternative promoter usage. These isoforms exhibit extracellular cadherin (EC) repeats that mediate calcium-dependent homophilic interactions, a transmembrane domain, and a conserved cytoplasmic tail involved in intracellular signaling.
PCDHgA3 plays a role in establishing neuronal identity and selective synaptic connections, contributing to the immense diversity of neural networks. Its expression is particularly prominent in the central nervous system, where it regulates axon guidance, dendritic arborization, and synapse formation. Dysregulation of PCDHgA3 has been implicated in neurodevelopmental disorders and cancers, highlighting its biological and clinical relevance.
Recombinant PCDHgA3 protein is engineered for in vitro studies to elucidate its structural and functional properties. Produced via expression systems like mammalian cells or *E. coli*, the purified protein retains key domains required for adhesion and signaling. Researchers utilize it to investigate binding partners, signaling pathways (e.g., Wnt/β-catenin), and mechanisms underlying neural circuit assembly. Additionally, it serves as a tool for developing diagnostic antibodies or exploring therapeutic strategies targeting PCDH-related pathologies.
Despite progress, the precise molecular roles of PCDHgA3 in health and disease remain incompletely understood. Ongoing studies aim to decode its interaction networks and validate its potential as a biomarker or drug target, bridging gaps in neurobiology and precision medicine.
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