| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITPR3 |
| Uniprot No | Q14573 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 304 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITPR3重组蛋白的3篇代表性文献，内容简明概括：
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1. **文献名称**：*Expression and Purification of Recombinant Human Inositol 1.4.5-Trisphosphate Receptor Type 3 (ITPR3) for Structural Studies*
**作者**：Smith A, et al.
**摘要**：报道了利用昆虫细胞表达系统成功表达并纯化人源ITPR3重组蛋白，通过冷冻电镜技术初步解析其跨膜结构域的构象特征，为研究其钙离子通道功能提供基础。
2. **文献名称**：*ITPR3-Mediated Calcium Signaling in Cancer Cell Proliferation*
**作者**：Zhang Y, et al.
**摘要**：研究发现ITPR3重组蛋白在多种癌细胞中异常高表达，通过调节内质网钙离子释放促进肿瘤细胞增殖，提示其作为潜在癌症治疗靶点的可能性。
3. **文献名称**：*Functional Characterization of ITPR3 Splice Variants Using Recombinant Protein Assays*
**作者**：Lee J, et al.
**摘要**：通过重组蛋白技术比较ITPR3不同剪接变体的功能差异，发现特定变体在钙振荡调控中具有独特动力学，可能与神经退行性疾病相关。
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注：以上文献为示例性内容，实际引用时需根据具体研究方向补充真实发表的论文(可通过PubMed或Web of Science检索关键词“ITPR3 recombinant protein”或“ITPR3 calcium signaling”获取)。
**Background of ITPR3 Recombinant Protein**
The inositol 1.4.5-trisphosphate receptor type 3 (ITPR3), a member of the IP3 receptor family, is a critical intracellular calcium (Ca²⁺) release channel located predominantly on the endoplasmic reticulum (ER). It plays a central role in Ca²⁺ signaling by mediating the release of Ca²⁺ from the ER into the cytosol upon binding to inositol 1.4.5-trisphosphate (IP3), a second messenger generated in response to extracellular stimuli such as hormones, neurotransmitters, or growth factors. ITPR3 is involved in regulating diverse cellular processes, including cell proliferation, apoptosis, metabolism, and gene expression. Dysregulation of ITPR3 has been implicated in pathologies like cancer, neurodegenerative disorders, and metabolic diseases.
Recombinant ITPR3 protein is engineered using genetic cloning techniques, often expressed in heterologous systems (e.g., mammalian or insect cell lines) to ensure proper post-translational modifications and functional fidelity. This engineered protein retains the structural domains essential for its activity: an N-terminal IP3-binding domain, a central regulatory region, and a C-terminal channel domain. Researchers utilize recombinant ITPR3 to study its biophysical properties, interaction partners, and modulation by ligands or drugs in vitro. It also serves as a tool for elucidating Ca²⁺ signaling mechanisms in diseases, screening therapeutic compounds targeting ITPR3-related pathways, and developing diagnostic assays. The production of recombinant ITPR3 enables precise, controlled experimentation, advancing our understanding of its physiological and pathological roles.
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