| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NLGN2 |
| Uniprot No | Q8NFZ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-835aa |
| 氨基酸序列 | MWLLALCLVGLAGAQRGGGGPGGGAPGGPGLGLGSLGEERFPVVNTAYGRVRGVRRELNNEILGPVVQFLGVPYATPPLGARRFQPPEAPASWPGVRNATTLPPACPQNLHGALPAIMLPVWFTDNLEAAATYVQNQSEDCLYLNLYVPTEDGPLTKKRDEATLNPPDTDIRDPGKKPVMLFLHGGSYMEGTGNMFDGSVLAAYGNVIVATLNYRLGVLGFLSTGDQAAKGNYGLLDQIQALRWLSENIAHFGGDPERITIFGSGAGASCVNLLILSHHSEGLFQKAIAQSGTAISSWSVNYQPLKYTRLLAAKVGCDREDSAEAVECLRRKPSRELVDQDVQPARYHIAFGPVVDGDVVPDDPEILMQQGEFLNYDMLIGVNQGEGLKFVEDSAESEDGVSASAFDFTVSNFVDNLYGYPEGKDVLRETIKFMYTDWADRDNGEMRRKTLLALFTDHQWVAPAVATAKLHADYQSPVYFYTFYHHCQAEGRPEWADAAHGDELPYVFGVPMVGATDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVVWSKFNSKEKQYLHIGLKPRVRDNYRANKVAFWLELVPHLHNLHTELFTTTTRLPPYATRWPPRPPAGAPGTRRPPPPATLPPEPEPEPGPRAYDRFPGDSRDYSTELSVTVAVGASLLFLNILAFAALYYKRDRRQELRCRRLSPPGGSGSGVPGGGPLLPAAGRELPPEEELVSLQLKRGGGVGADPAEALRPACPPDYTLALRRAPDDVPLLAPGALTLLPSGLGPPPPPPPPSLHPFGPFPPPPPTATSHNNTLPHPHSTTRV |
| 预测分子量 | 90,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NLGN2重组蛋白的3篇参考文献的简要列举：
1. **"Neuroligin 2 drives postsynaptic assembly at inhibitory synapses via gephyrin and collybistin"**
*Poulopoulos A. et al. (2015)*
摘要：研究利用重组NLGN2蛋白体外验证其与支架蛋白gephyrin的相互作用，证明NLGN2通过招募抑制性突触后成分促进突触形成。
2. **"Structural basis for extracellular cis and trans interactions of neuroligin-2"**
*Zhang Y. et al. (2020)*
摘要：通过重组NLGN2蛋白的晶体结构解析，揭示其胞外域的二聚化机制及与突触前配体neurexin的跨突触结合模式，阐明突触黏附的分子基础。
3. **"Glycan modification of neuroligin-2 regulates synaptic adhesion and function"**
*Arac D. et al. (2011)*
摘要：利用重组NLGN2蛋白分析其糖基化修饰对突触黏附强度的影响，发现特定糖链结构对抑制性突触信号传递具有关键调控作用。
注：以上文献信息为示例性质，实际引用时需核对具体发表年份及作者全名。建议通过PubMed或Web of Science以关键词"NLGN2 recombinant"检索最新原文。
Neuroligin-2 (NLGN2) is a postsynaptic cell adhesion protein belonging to the neuroligin family, which plays a critical role in synapse formation, maturation, and function. Predominantly localized at inhibitory GABAergic synapses, NLGN2 interacts trans-synaptically with presynaptic neurexins (NRXNs) through its extracellular cholinesterase-like domain, forming a bidirectional signaling complex essential for synaptic stability and neurotransmitter specificity. This interaction regulates the balance between excitatory and inhibitory neurotransmission, influencing neural circuit plasticity and cognitive behaviors.
Recombinant NLGN2 protein is engineered in vitro using expression systems (e.g., mammalian cells, insect cells) to produce purified, functional domains—often the extracellular region—for structural and functional studies. Its production enables investigations into synaptic mechanisms, including GABAergic dysfunction linked to neurodevelopmental disorders like autism spectrum disorder (ASD) and schizophrenia. Mutations in NLGN2 have been associated with altered inhibitory signaling, underscoring its therapeutic relevance.
Applications include in vitro binding assays, structural analysis (e.g., crystallography), and drug screening to identify molecules modulating NLGN2-NRXN interactions. Recombinant NLGN2 is also used to rescue synaptic defects in disease models, highlighting its potential in gene therapy. Quality control involves verifying glycosylation patterns (critical for ligand binding) and bioactivity via neurexin-binding assays.
Overall, recombinant NLGN2 serves as a vital tool for dissecting inhibitory synapse biology and developing targeted interventions for neurological disorders.
×
