| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP2a1 |
| Uniprot No | O95782 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-977aa |
| 氨基酸序列 | MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF |
| 预测分子量 | 107,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP2A1重组蛋白的3篇参考文献示例(文献信息为虚构，仅供格式参考)：
1. **《Recombinant AP2A1 protein purification and DNA-binding activity analysis》**
Smith, J. et al. (2015)
摘要：该研究成功在大肠杆菌中表达并纯化了重组AP2A1蛋白，通过电泳迁移实验(EMSA)证实其特异性结合靶基因启动子区域的能力，为后续功能研究提供基础材料。
2. **《Structural characterization of human AP2A1 using X-ray crystallography》**
Lee, H. et al. (2020)
摘要：作者利用重组AP2A1蛋白进行结晶和X射线衍射分析，首次解析了其三维结构，揭示了该蛋白与DNA相互作用的关键结构域，为设计靶向药物提供理论依据。
3. **《AP2A1 recombinant protein inhibits tumor growth in triple-negative breast cancer models》**
Zhang, Y. et al. (2018)
摘要：通过体外重组AP2A1蛋白处理乳腺癌细胞，发现其能显著下调癌基因表达并诱导细胞凋亡，动物实验显示重组蛋白可抑制肿瘤生长，提示其潜在治疗价值。
(注：实际文献需通过PubMed、Web of Science等学术数据库检索获取。)
**Background of AP2a1 Recombinant Protein**
AP2a1. also known as Transcription Factor AP-2 Alpha (TFAP2A), is a member of the AP-2 transcription factor family, which plays critical roles in embryonic development, cell proliferation, and differentiation. This protein is characterized by a helix-span-helix dimerization domain and a basic DNA-binding region, enabling it to regulate gene expression by binding to specific DNA sequences. AP2a1 is particularly vital in neural crest cell development, epidermal formation, and organogenesis, with mutations linked to developmental disorders such as branchiooculofacial syndrome.
Recombinant AP2a1 protein is engineered *in vitro* using expression systems like *E. coli* or mammalian cells, ensuring high purity and activity for research applications. Its production typically involves cloning the TFAP2A gene into a plasmid vector, followed by transfection, expression, and purification via affinity chromatography (e.g., His-tag or GST-tag systems).
In research, AP2a1 recombinant protein serves as a tool to study transcriptional regulation, cell lineage specification, and disease mechanisms. It is widely used in electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and functional studies to explore its role in cancers, where AP-2 family members often exhibit dysregulated expression. Additionally, it aids in drug discovery by screening molecules that modulate AP2a1 activity.
The availability of recombinant AP2a1 has advanced understanding of its structural and functional dynamics, offering insights into developmental biology and therapeutic targeting for related pathologies.
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