| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHH |
| Uniprot No | O43323 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-198aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMIIGPGRGPVGRRRYARKQLVPLLYKQ FVPGVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADR LMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRAL DITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAG G |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DHH(Desert Hedgehog)重组蛋白的模拟参考文献示例(注：内容为虚构，仅作格式参考)：
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1. **文献名称**: "Expression and Purification of Recombinant DHH Protein in Mammalian Cells"
**作者**: Smith, J. et al.
**摘要**: 本研究报道了一种在HEK293细胞中高效表达并纯化具有生物活性的重组DHH蛋白的方法。通过优化信号肽和糖基化修饰，纯化的DHH蛋白成功激活Hedgehog通路下游靶基因，为体外功能研究提供了工具。
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2. **文献名称**: "DHH Recombinant Protein Promotes Schwann Cell Differentiation in Vitro"
**作者**: Chen, L. & Tanaka, K.
**摘要**: 利用大肠杆菌表达系统制备的重组DHH蛋白，验证了其对神经嵴来源的施万细胞分化的调控作用，揭示了DHH通过结合Patched-1受体激活Gli转录因子的分子机制。
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3. **文献名称**: "Therapeutic Potential of DHH in Testicular Development Disorders"
**作者**: Gupta, R. et al.
**摘要**: 通过注射重组DHH蛋白至小鼠模型，发现其可挽救因DHH基因突变导致的睾丸支持细胞功能障碍，表明重组DHH可能作为生殖系统发育异常的治疗策略。
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4. **文献名称**: "Crystal Structure Analysis of Recombinant DHH and Its Interaction with Dispatched Homolog"
**作者**: Wang, Y. et al.
**摘要**: 解析了重组DHH蛋白的晶体结构，并揭示其与转运蛋白Dispatched的结合域，为设计靶向Hedgehog信号通路的小分子药物提供了结构基础。
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如需真实文献，建议通过PubMed、Google Scholar等平台以“DHH recombinant protein”或“Desert Hedgehog expression”为关键词检索。
**Background of DHH Recombinant Protein**
The Desert Hedgehog (DHH) protein is a member of the Hedgehog (Hh) signaling family, which plays critical roles in embryonic development, tissue homeostasis, and cellular differentiation. Discovered in the 1990s, the Hh family comprises three mammalian homologs: Sonic Hedgehog (SHH), Indian Hedgehog (IHH), and Desert Hedgehog (DHH). DHH, the least studied among the three, is primarily expressed in gonadal tissues, Schwann cells, and peripheral nerves, where it regulates processes such as testicular development, spermatogenesis, and myelination of peripheral nerves.
Recombinant DHH protein is engineered through genetic engineering techniques, where the DHH gene is cloned into expression vectors and produced in host systems like *E. coli* or mammalian cell lines. This allows for large-scale production of purified, biologically active DHH, essential for research and therapeutic applications. Recombinant DHH retains the ability to bind its receptor Patched-1 (PTCH1), initiating downstream signaling via Smoothened (SMO) and GLI transcription factors, mimicking endogenous Hh pathway activation.
Studies using DHH recombinant protein have highlighted its role in maintaining the blood-testis barrier, supporting Leydig cell function, and promoting Schwann cell differentiation. Deficiencies or mutations in DHH are linked to disorders such as gonadal dysgenesis and peripheral neuropathies. In regenerative medicine, recombinant DHH is explored for nerve repair and fertility treatments. Its application in drug discovery includes screening for agonists or antagonists to modulate Hh signaling, which is often dysregulated in cancers and developmental disorders.
Overall, DHH recombinant protein serves as a vital tool for dissecting Hh signaling mechanisms and developing targeted therapies, bridging gaps in understanding reproductive biology and neural physiology.
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