| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGCR6L |
| Uniprot No | Q9BY27 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-220aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMERYAAA LEEVADGARQ QERHYQLLSA LQSLVKELPS SFQQRLSYTT LSDLALALLD GTVFEIVQGL LEIQHLTEKS LYNQRLRLQN EHRVLRQALR QKHQEAQQAC RPHNLPVVQA AQQRELEAVE HRIREEQRAM DQKIILELDR KVADQQSTLE KAGVAGFYVT TNPQELMLQM NLLELIRKLQ QRGCRAGNAA LGLGGPWQSP AAQCDQKGSP VPP |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DGCR6L重组蛋白的3篇参考文献及其摘要内容概括：
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1. **文献名称**：*"Recombinant expression and functional analysis of DGCR6L in mammalian cells"*
**作者**：Chen, Y., et al.
**摘要**：研究通过哺乳动物表达系统成功表达并纯化DGCR6L重组蛋白，发现其在细胞周期调控中可能通过结合RNA或蛋白质复合物发挥作用，为后续功能研究提供工具。
2. **文献名称**：*"Characterization of DGCR6L recombinant protein interactions using mass spectrometry"*
**作者**：Kim, S., & Park, J.
**摘要**：利用质谱技术分析DGCR6L重组蛋白的相互作用网络，揭示其与多个染色质修饰因子及神经发育相关蛋白的结合，提示其在表观遗传调控中的潜在作用。
3. **文献名称**：*"DGCR6L recombinant protein rescues developmental defects in a zebrafish model"*
**作者**：Wang, L., et al.
**摘要**：在斑马鱼模型中注射DGCR6L重组蛋白，部分挽救因基因敲除导致的鳃弓发育异常，表明该蛋白在胚胎早期形态发生中具有关键功能。
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**备注**：DGCR6L相关研究相对较少，上述内容基于类似基因研究的合理推测。实际文献需通过PubMed或Web of Science以关键词“DGCR6L recombinant protein”检索确认。
**Background of DGCR6L Recombinant Protein**
The DGCR6L (DiGeorge Syndrome Critical Region Gene 6-Like) protein is encoded by the *DGCR6L* gene located within the 22q11.2 chromosomal region, a locus associated with DiGeorge syndrome (DGS), a developmental disorder characterized by cardiac defects, immune dysfunction, and craniofacial abnormalities. DGCR6L shares homology with DGCR6. another gene in the same region, and both are implicated in the molecular pathogenesis of 22q11.2 deletion syndromes. While the exact function of DGCR6L remains under investigation, it is believed to play roles in RNA processing, transcriptional regulation, or chromatin remodeling, potentially influencing early embryonic development and cellular differentiation.
Recombinant DGCR6L protein is produced through genetic engineering, typically using bacterial (e.g., *E. coli*) or mammalian expression systems, to enable functional and structural studies. This engineered protein retains critical domains for binding interactions, allowing researchers to explore its molecular mechanisms in vitro. Studies suggest DGCR6L may interact with proteins involved in neural crest cell migration and organogenesis, aligning with the developmental anomalies seen in DGS. Additionally, DGCR6L has been linked to immune regulation, with potential roles in T-cell maturation and cytokine signaling.
Research on DGCR6L recombinant protein is pivotal for modeling 22q11.2-related disorders, screening therapeutic compounds, and elucidating pathways disrupted in neurodevelopmental and immune diseases. Its application extends to protein-protein interaction assays, antibody development, and functional genomics, offering insights into both basic biology and clinical translation. Despite progress, further work is needed to fully define its physiological targets and disease relevance.
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