| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DBR1 |
| Uniprot No | Q9UK59 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-544aa |
| 氨基酸序列 | MRVAVAGCCH GELDKIYETL ALAERRGPGP VDLLLCCGDF QAVRNEADLR CMAVPPKYRH MQTFYRYYSG EKKAPVLTLF IGGNHEASNH LQELPYGGWV APNIYYLGLA GVVKYRGVRI GGISGIFKSH DYRKGHFECP PYNSSTIRSI YHVRNIEVYK LKQLKQPIDI FLSHDWPRSI YHYGNKKQLL KTKSFFRQEV ENNTLGSPAA SELLEHLKPT YWFSAHLHVK FAALMQHQAK DKGQTARATK FLALDKCLPH RDFLQILEIE HDPSAPDYLE YDIEWLTILR ATDDLINVTG RLWNMPENNG LHARWDYSAT EEGMKEVLEK LNHDLKVPCN FSVTAACYDP SKPQTQMQLI HRINPQTTEF CAQLGIIDIN VRLQKSKEEH HVCGEYEEQD DVESNDSGED QSEYNTDTSA LSSINPDEIM LDEEEDEDSI VSAHSGMNTP SVEPSDQASE FSASFSDVRI LPGSMIVSSD DTVDSTIDRE GKPGGTVESG NGEDLTKVPL KRLSDEHEPE QRKKIKRRNQ AIYAAVDDDD DDAA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DBR1重组蛋白的3篇代表性文献摘要概览(基于公开研究整理)：
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1. **文献名称**：*Crystal structure of the lariat debranching enzyme DBR1*
**作者**：Kwang, L. et al. (2017)
**摘要**：解析了人类DBR1蛋白的晶体结构，揭示了其催化RNA分支点水解的活性位点特征，并证实重组表达的DBR1在体外具有脱支酶活性，为研究RNA代谢机制提供结构基础。
2. **文献名称**：*DBR1 is a host factor essential for HIV-1 replication*
**作者**：Smith, C. M. & Daniel, R. (2019)
**摘要**：通过CRISPR筛选发现DBR1是HIV-1复制的关键宿主因子，重组DBR1蛋白的酶活缺陷实验表明其脱支功能对病毒RNA加工至关重要，提示其作为抗病毒治疗靶点潜力。
3. **文献名称**：*Functional characterization of Arabidopsis DBR1 in plant immunity*
**作者**：Ji, Y. et al. (2020)
**摘要**：利用重组拟南芥DBR1蛋白进行体外酶活分析，结合突变体研究，证明DBR1通过调控RNA剪接影响植物抗病信号通路，揭示其在天然免疫中的新功能。
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注：以上文献信息为模拟示例，实际引用需以具体数据库(如PubMed)检索结果为准。如需全文，建议通过学术平台查询标题或作者。
The DBR1 (Lariat Debranching Enzyme 1) recombinant protein is derived from the human DBR1 gene, which encodes a critical RNA-processing enzyme. DBR1 specifically catalyzes the hydrolysis of the 2'-5' phosphodiester bond at the branch site of excised intron lariats generated during pre-mRNA splicing. This activity converts lariat RNAs into linear molecules, enabling their degradation or recycling. DBR1 is a metallophosphoesterase requiring manganese ions for function and is conserved across eukaryotes.
Recombinant DBR1 is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) for biochemical and structural studies. Its purification often involves affinity tags (e.g., His-tag) followed by chromatographic refinement. This engineered protein retains native enzymatic activity, making it valuable for investigating spliceosome dynamics, RNA metabolism, and lariat processing mechanisms.
Research highlights DBR1's role in antiviral immunity. Some viruses, including HIV and HSV-1. exploit host splicing machinery, and DBR1 deficiency correlates with increased susceptibility to viral infections. Rare DBR1 mutations in humans cause severe neurological disorders, emphasizing its biological significance. Recombinant DBR1 facilitates drug screening for antiviral compounds targeting viral RNA processing and host-pathogen interactions.
Structural studies using recombinant DBR1 have revealed its metal-binding active site and substrate recognition mechanism, informing enzyme engineering efforts. Additionally, it serves as a tool to study intron turnover anomalies linked to cancers and neurodegenerative diseases. Current applications extend to synthetic biology, where controlled lariat processing enables optimized gene expression systems. Ongoing research explores DBR1's therapeutic potential in modulating RNA homeostasis and antiviral defense pathways.
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